학술논문
Cross-reactivity of hantavirus antibodies after immunization with PUUV antigens.
Document Type
Academic Journal
Author
Shkair L; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.; Sharma D; Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, India.; Hamza S; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.; Garanina E; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.; Shakirova V; Department of Infectious Diseases, Kazan State Medical Academy, Kazan, Russia.; Khaertynova I; Department of Infectious Diseases, Kazan State Medical Academy, Kazan, Russia.; Markelova M; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.; Pavelkina V; Infectious Diseases Department, National Research Ogarev Mordovia State University, Saransk, Russia.; Rizvanov A; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.; Khaiboullina S; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.; Baranwal M; Infectious Diseases Department, National Research Ogarev Mordovia State University, Saransk, Russia.; Martynova E; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
Source
Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8609465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1470-8744 (Electronic) Linking ISSN: 08854513 NLM ISO Abbreviation: Biotechnol Appl Biochem Subsets: MEDLINE
Subject
Language
English
Abstract
Nephropathia epidemica (NE), caused by Puumala (PUUV) orthohantavirus, is endemic in the Republic of Tatarstan (RT). There are limited options for NE prevention in RT. Currently, available vaccines are made using Haantan (HNTV) orthohantavirus antigens. In this study, the efficacy of microvesicles (MVs) loaded with PUUV antigens to induce the humoral immune response in small mammals was analyzed. Additionally, the cross-reactivity of serum from immunized small mammals and NE patients with HNTV, Dobrava, and Andes orthohantaviruses was investigated using nucleocapsid (N) protein peptide libraries. Finally, the selected peptides were analyzed for allergenicity, their ability to induce an autoimmune response, and their interaction with Class II HLA. Several N protein peptides were found to be cross-reactive with serum from MVs immunized small mammals. These cross-reactive epitopes were located in oligomerization perinuclear targeting and Daxx-interacting domains. Most cross-reactive peptides lack allergenic and autoimmune reactivity. Molecular docking revealed two cross-reacting peptides, N6 and N19, to have good binding with three Class II HLA alleles. These peptides could be candidates for developing vaccines and therapeutics for NE.
(© 2024 International Union of Biochemistry and Molecular Biology, Inc.)
(© 2024 International Union of Biochemistry and Molecular Biology, Inc.)