학술논문
Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children.
Document Type
Academic Journal
Author
Córdova S; Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain.; Tena-Garitaonaindia M; Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain.; Álvarez-Mercado AI; Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain.; Ibs.GRANADA, 18012 Granada, Spain.; Gámez-Belmonte R; Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain.; Gómez-Llorente MA; Ibs.GRANADA, 18012 Granada, Spain.; Unidad de Pediatría, Hospital Materno-Infantil, 18071 Granada, Spain.; Sánchez de Medina F; Ibs.GRANADA, 18012 Granada, Spain.; Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain.; Centro de Investigación Biomédica en Red-Enfermedades Hepáticas y Digestivas (CIBERehd), Spain.; Martínez-Cañavate A; Unidad de Pediatría, Hospital Materno-Infantil, 18071 Granada, Spain.; Martínez-Augustin O; Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain.; Ibs.GRANADA, 18012 Granada, Spain.; Centro de Investigación Biomédica en Red-Enfermedades Hepáticas y Digestivas (CIBERehd), Spain.; Gómez-Llorente C; Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, Spain.; Ibs.GRANADA, 18012 Granada, Spain.; Instituto de Nutrición y Tecnología de los Alimento José Mataix, 18071 Granada, Spain.; Centro de Investigación Biomédica en Red-Obesidad (CIBERobn), Spain.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
Subject
Language
English
Abstract
Asthma is a multifactorial condition that can be associated with obesity. The phenotypes of asthma in lean and obese patients are different, with proinflammatory signatures being further elevated in the latter. Both obesity and asthma are associated with alterations in intestinal barrier function and immunity, and with the composition of the intestinal microbiota and food consumption. In this study, we aimed to establish an organoid model to test the hypothesis that the intestinal content of lean and obese, allergic, asthmatic children differentially regulates epithelial intestinal gene expression. A model of mouse jejunum intestinal organoids was used. A group of healthy, normal-weight children was used as a control. The intestinal content of asthmatic obese children differentially induced the expression of inflammatory and mitochondrial response genes ( Tnf -tumor necrosis factor, Cd14 , Muc13 -mucin 13, Tff2 -Trefoil factor 2 and Tff3 , Cldn1 -claudin 1 and 5 , Reg3g -regenerating family member 3 gamma, mt-Nd1 -NADH dehydrogenase 1 and 6 , and mt-Cyb -mitochondrial cytochrome b) via the RAGE-advanced glycosylation end product-specific receptor, NF-κB-nuclear factor kappa b and AKT kinase signal transduction pathways. Fecal homogenates from asthmatic normal-weight and obese children induce a differential phenotype in intestinal organoids, in which the presence of obesity plays a major role.