학술논문
Protein Analysis of A. halleri and N. caerulescens Hyperaccumulators When Exposed to Nano and Ionic Forms of Cd and Zn.
Document Type
Academic Journal
Author
Gallo V; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43126 Parma, Italy.; Serianni VM; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43126 Parma, Italy.; Imperiale D; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43126 Parma, Italy.; Experimental Station for the Food Preservation Industry-Research Foundation, 43121 Parma, Italy.; Zappettini A; Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), 06128 Parma, Italy.; Villani M; Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), 06128 Parma, Italy.; Marmiroli M; Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43126 Parma, Italy.; Marmiroli N; The Italian National Interuniversity Consortium for Environmental Sciences (CINSA), 43124 Parma, Italy.
Source
Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101610216 Publication Model: Electronic Cited Medium: Print ISSN: 2079-4991 (Print) Linking ISSN: 20794991 NLM ISO Abbreviation: Nanomaterials (Basel) Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2079-4991
Abstract
Hyperaccumulator plant species growing on metal-rich soils can accumulate high quantity of metals and metalloids in aerial tissues, and several proteomic studies on the molecular mechanisms at the basis of metals resistance and hyperaccumulation have been published. Hyperaccumulator are also at the basis of the phytoremediation strategy to remove metals more efficiently from polluted soils or water. Arabidopsis halleri and Noccea caerulescens are both hyperaccumulators of metals and nano-metals. In this study, the change in some proteins in A. halleri and N. caerulescens was assessed after the growth in soil with cadmium and zinc, provided as sulphate salts (CdSO 4 and ZnSO 4 ) or sulfide quantum dots (CdS QDs and ZnS QDs). The protein extracts obtained from plants after 30 days of growth were analyzed by 2D-gel electrophoresis (2D SDS-PAGE) and identified by MALDI-TOF/TOF mass spectrometry. A bioinformatics analysis was carried out on quantitative protein differences between control and treated plants. In total, 43 proteins resulted in being significatively modulated in A. halleri , while 61 resulted in being modulated in N. caerulescens . Although these two plants are hyperaccumulator of both metals and nano-metals, at protein levels the mechanisms involved do not proceed in the same way, but at the end bring a similar physiological result.