학술논문
Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro.
Document Type
Academic Journal
Author
Paudel KR; School of Life Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia.; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.; Mehta M; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia.; Yin GHS; School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.; Yen LL; School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.; Malyla V; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia.; Patel VK; School of Life Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia.; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.; Panneerselvam J; Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.; Madheswaran T; Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.; MacLoughlin R; IDA Business Park, Dangan, H91 HE94, Galway, Ireland.; School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland, Dublin, D02 YN77, Ireland.; School of Pharmacy & Pharmaceutical Sciences, Trinity College, Dublin, D02 PN40, Ireland.; Jha NK; Department of Biotechnology, School of Engineering & Technology (SET), Sharda University, Greater Noida, Uttar Pradesh, 201310, India.; Gupta PK; Department of Life Sciences, School of Basic Sciences and Research (SBSR), Sharda University, Knowledge Park III, Greater Noida-201310, Uttar Pradesh, India.; Singh SK; School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, 144411, India.; Gupta G; School of Pharmacy, Suresh Gyan Vihar University, Jagatpura 302017, Mahal Road, Jaipur, India.; Kumar P; Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 2193, South Africa.; Oliver BG; School of Life Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia.; Woolcock Institute of Medical Research, University of Sydney, Sydney, NSW, 2006, Australia.; Hansbro PM; School of Life Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia. Kamal.Dua@uts.edu.au.; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia. Kamal.Dua@uts.edu.au.; Chellappan DK; Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia. Kamal.Dua@uts.edu.au.; Dua K; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia. Kamal.Dua@uts.edu.au.; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW, 2007, Australia. Kamal.Dua@uts.edu.au.; Woolcock Institute of Medical Research, University of Sydney, Sydney, NSW, 2006, Australia. Kamal.Dua@uts.edu.au.; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW, 2007, Australia. Kamal.Dua@uts.edu.au.
Source
Publisher: Springer Country of Publication: Germany NLM ID: 9441769 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1614-7499 (Electronic) Linking ISSN: 09441344 NLM ISO Abbreviation: Environ Sci Pollut Res Int Subsets: MEDLINE
Subject
Language
English
Abstract
Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we formulated berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated their in vitro antiproliferative and antimigratory activity in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were carried out to determine the anti-migratory activity. We also investigated major proteins associated with lung cancer progression. The developed nanoparticles were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35%) and have shown sustained release behaviour. The most remarkable findings reported with berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration via epithelial mesenchymal transition, and proliferation related proteins associated with cancer progression. Our findings suggest that anti-cancer compounds with the problem of poor solubility and bioavailability can be overcome by formulating them into nanotechnology-based delivery systems for better efficacy. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of berberine-LCNs as a potential NSCLC treatment option.
(© 2022. The Author(s).)
(© 2022. The Author(s).)