학술논문
Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro.
Document Type
Academic Journal
Author
Mehta M; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, New South Wales, Australia.; Centre for Inflammation, Centenary Institute, Sydney, New South Wales, Australia.; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, New South Wales, Australia.; Malyla V; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, New South Wales, Australia.; Centre for Inflammation, Centenary Institute, Sydney, New South Wales, Australia.; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, New South Wales, Australia.; Paudel KR; Centre for Inflammation, Centenary Institute, Sydney, New South Wales, Australia.; School of Life Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.; Chellappan DK; Department of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia.; Hansbro PM; Centre for Inflammation, Centenary Institute, Sydney, New South Wales, Australia.; School of Life Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.; Oliver BG; School of Life Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.; Woolcock Institute of Medical Research, University of Sydney, Sydney, New South Wales, Australia.; Dua K; Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, New South Wales, Australia.; Centre for Inflammation, Centenary Institute, Sydney, New South Wales, Australia.; Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, New South Wales, Australia.
Source
Publisher: Wiley Country of Publication: United States NLM ID: 7706045 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1745-4514 (Electronic) Linking ISSN: 01458884 NLM ISO Abbreviation: J Food Biochem Subsets: MEDLINE
Subject
Language
English
Abstract
Metastasis represents the leading cause of death in lung cancer patients. C-X-C Motif Chemokine Ligand 8 (CXCL-8), Chemokine (C-C motif) ligand 20 (CCL-20) and heme oxygenase -1 (HO-1) play an important role in cancer cell proliferation and migration. Berberine is an isoquinoline alkaloid isolated from several herbs in the Papaveraceae family that exhibits anti-inflammatory, anticancer and antidiabetic properties. Therefore, the aim of present study is to investigate the inhibitory potential of berberine monoolein loaded liquid crystalline nanoparticles (berberine-LCNs) against cancer progression. Berberine-LCNs were prepared by mixing berberine, monoolein and poloxamer 407 (P407) using ultrasonication method. A549 cells were treated with or without 5 µM dose of berberine LCNs for 24 hr and total cellular protein was extracted and further analyzed for the protein expression of CCl-20, CXCL-8 and HO-1 using human oncology array kit. Our results showed that berberine-LCNs significantly reduced the expression of CCl-20, CXCL-8 and HO-1 at dose of 5µM. Collectively, our findings suggest that berberine-LCNs have inhibitory effect on inflammation/oxidative stress related cytokines i.e. CCL20, CXCL-8, and HO-1 which could be a novel therapeutic target for the management of lung cancer. PRACTICAL APPLICATIONS: Berberine is an isoquinoline alkaloid extracted from various plants of Papaveraceae family. CXCL-8, CCL-20 and HO-1 play an important role in cancer progression. Our study showed that Berberine LCNs significantly downregulate the expression of CXCL-8, CCL-20 and HO-1 which suggests that Berberine loaded nanoparticles could be a promising therapeutic alternative for the management of lung cancer.
(© 2021 Wiley Periodicals LLC.)
(© 2021 Wiley Periodicals LLC.)