학술논문
High-Throughput Protein-Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline.
Document Type
Academic Journal
Author
Castandet B; Institute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, Université Paris-Cité, CNRS, INRAE, Université Evry, Gif sur Yvette, France.; Lurin C; Institute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, Université Paris-Cité, CNRS, INRAE, Université Evry, Gif sur Yvette, France.; Delannoy É; Institute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, Université Paris-Cité, CNRS, INRAE, Université Evry, Gif sur Yvette, France.; Monachello D; Institute of Plant Sciences Paris-Saclay (IPS2), Université Paris-Saclay, Université Paris-Cité, CNRS, INRAE, Université Evry, Gif sur Yvette, France. dario.monachello@inrae.fr.
Source
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Subject
Language
English
Abstract
Because of its adaptability to high-throughput approaches and a low operating cost, the yeast two-hybrid (Y2H) assay remains the most widely used one for high-throughput protein-protein interactions (PPI) mapping experiments. Here we provide a detailed protocol for a liquid culture-based high-throughput binary protein-protein Y2H screen pipeline of a pool of 50 proteins used as baits against a collection of ~12,000 Arabidopsis proteins encoded by sequence-verified open reading frames (ORFs).
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)