학술논문
Clinical Significance of Stem Cell Biomarkers EpCAM, LGR5 and LGR4 mRNA Levels in Lymph Nodes of Colon Cancer Patients.
Document Type
Academic Journal
Author
AbdelMageed M; Department of Clinical Microbiology, Infection and Immunology, Umeå University, SE-90185 Umeå, Sweden.; Department of Radiation Sciences, Oncology, Umeå University, SE-90185 Umeå, Sweden.; Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.; Ismail HTH; Department of Clinical Microbiology, Infection and Immunology, Umeå University, SE-90185 Umeå, Sweden.; Department of Radiation Sciences, Oncology, Umeå University, SE-90185 Umeå, Sweden.; Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.; Olsson L; Department of Clinical Microbiology, Infection and Immunology, Umeå University, SE-90185 Umeå, Sweden.; Lindmark G; Institution of Clinical Sciences, Lund University, SE-25187 Helsingborg, Sweden.; Hammarström ML; Department of Clinical Microbiology, Infection and Immunology, Umeå University, SE-90185 Umeå, Sweden.; Hammarström S; Department of Clinical Microbiology, Infection and Immunology, Umeå University, SE-90185 Umeå, Sweden.; Sitohy B; Department of Clinical Microbiology, Infection and Immunology, Umeå University, SE-90185 Umeå, Sweden.; Department of Radiation Sciences, Oncology, Umeå University, SE-90185 Umeå, Sweden.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
Subject
Language
English
Abstract
The significance of cancer stem cells (CSCs) in initiation and progression of colon cancer (CC) has been established. In this study, we investigated the utility of measuring mRNA expression levels of CSC markers EpCAM, LGR5 and LGR4 for predicting survival outcome in surgically treated CC patients. Expression levels were determined in 5 CC cell lines, 66 primary CC tumors and 382 regional lymph nodes of 121 CC patients. Prognostic relevance was determined using Kaplan-Meier survival and Cox regression analyses. CC patients with lymph nodes expressing high levels of EpCAM, LGR5 or LGR4 (higher than a clinical cutoff of 0.07, 0.06 and 2.558 mRNA copies/18S rRNA unit, respectively) had a decreased mean survival time of 32 months for EpCAM and 42 months for both LGR5 and LGR4 at a 12-year follow-up ( p = 0.022, p = 0.005 and p = 0.011, respectively). Additional patients at risk for recurrence were detected when LGR5 was combined with the biomarkers CXCL17 or CEA plus CXCL16. In conclusion, the study underscores LGR5 as a particularly useful prognostic biomarker and illustrates the strength of combining biomarkers detecting different subpopulations of cancer cells and/or cells in the tumor microenvironment for predicting recurrence.