학술논문
Protocol for the Investigation of Plasma and Whole Blood Clot Property of Fibrin Fiber Thickness Using Scanning Electron Microscopy.
Document Type
Academic Journal
Author
Cai T; Murdoch Children's Research Institute, Parkville, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, Australia.; McCafferty C; Murdoch Children's Research Institute, Parkville, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, Australia.; Van Den Helm S; Murdoch Children's Research Institute, Parkville, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, Australia.; Letunica N; Murdoch Children's Research Institute, Parkville, Australia.; Attard C; Murdoch Children's Research Institute, Parkville, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, Australia.; Monagle P; Murdoch Children's Research Institute, Parkville, Australia.; Department of Paediatrics, The University of Melbourne, Parkville, Australia.; The Royal Children's Hospital, Parkville, Australia.; Ignjatovic V; Murdoch Children's Research Institute, Parkville, Australia. verai@unimelb.edu.au.; Department of Paediatrics, The University of Melbourne, Parkville, Australia. verai@unimelb.edu.au.
Source
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Subject
Language
English
Abstract
Blood clot formation represents a key component of the coagulation process for preventing excessive hemorrhage. The structural characteristics of blood clots are associated with their strength and susceptibility to fibrinolysis. Scanning electron microscopy is a technique that allows for state-of-the-art image capture of blood clots, providing visualization of topography, fibrin thickness, fibrin network density, and blood cell involvement and morphology. In this chapter, we provide a detailed protocol for characterization of plasma and whole blood clot structure using SEM, covering the spectrum from blood collection, in vitro clot formation, sample preparation for SEM, imaging, and image analysis, specifically focusing on the measurement of fibrin fiber thickness.
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)