학술논문
Microbiota from human infants consuming secretors or non-secretors mothers' milk impacts the gut and immune system in mice.
Document Type
Academic Journal
Author
Gurung M; Microbiome and Metabolism Research Unit (MMRU), USDA-ARS, SEA, Arkansas Children's Nutrition Center, Little Rock, Arkansas, USA.; Schlegel BT; University of Pittsburgh Medical Center (UPMC), Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Rajasundaram D; University of Pittsburgh Medical Center (UPMC), Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA.; Fox R; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.; Bode L; Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California San Diego, La Jolla, California, USA.; Department of Pediatrics, University of California San Diego, La Jolla, California, USA.; Yao T; Department of Food Science, Whistler Center for Carbohydrate Research, Purdue University, West Lafayette, Indiana, USA.; Lindemann SR; Department of Food Science, Whistler Center for Carbohydrate Research, Purdue University, West Lafayette, Indiana, USA.; LeRoith T; Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, Virginia, USA.; Read QD; USDA-ARS, SEA, Raleigh, North Carolina, USA.; Simecka C; Division of Laboratory Animal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.; Carroll L; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.; Andres A; Microbiome and Metabolism Research Unit (MMRU), USDA-ARS, SEA, Arkansas Children's Nutrition Center, Little Rock, Arkansas, USA.; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.; Yeruva L; Microbiome and Metabolism Research Unit (MMRU), USDA-ARS, SEA, Arkansas Children's Nutrition Center, Little Rock, Arkansas, USA.; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Source
Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-5077 (Electronic) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: MEDLINE
Subject
Language
English
Abstract
Maternal secretor status is one of the determinants of human milk oligosaccharides (HMOs) composition, which, in turn, influences the gut microbiota composition of infants. To understand if this change in gut microbiota impacts immune cell composition, intestinal morphology, and gene expression, 21-day-old germ-free C57BL/6 mice were transplanted with fecal microbiota from infants whose mothers were either secretors (SMM) or non-secretors (NSM) or from infants consuming dairy-based formula (MFM). For each group, one set of mice was supplemented with HMOs. HMO supplementation did not significantly impact the microbiota diversity; however, SMM mice had a higher abundance of genus Bacteroides , Bifidobacterium , and Blautia , whereas, in the NSM group, there was a higher abundance of Akkermansia , Enterocloster , and Klebsiella . In MFM, gut microbiota was represented mainly by Parabacteroides , Ruminococcaceae_unclassified , and Clostrodium_sensu_stricto . In mesenteric lymph node, Foxp3+ T cells and innate lymphoid cells type 2 were increased in MFM mice supplemented with HMOs, while in the spleen, they were increased in SMM + HMOs mice. Similarly, serum immunoglobulin A was also elevated in MFM + HMOs group. Distinct global gene expression of the gut was observed in each microbiota group, which was enhanced with HMOs supplementation. Overall, our data show that distinct infant gut microbiota due to maternal secretor status or consumption of dairy-based formula and HMO supplementation impacts immune cell composition, antibody response, and intestinal gene expression in a mouse model.
Importance: Early life factors like neonatal diet modulate gut microbiota, which is important for the optimal gut and immune function. One such factor, human milk oligosaccharides (HMOs), the composition of which is determined by maternal secretor status, has a profound effect on infant gut microbiota. However, how the infant gut microbiota composition determined by maternal secretor status or consumption of infant formula devoid of HMOs impacts infant intestinal ammorphology, gene expression, and immune signature is not well explored. This study provides insights into the differential establishment of infant microbiota derived from infants fed by secretor or non-secretor mothers milk or those consuming infant formula and demonstrates that the secretor status of mothers promotes Bifidobacteria and Bacteroides sps. establishment. This study also shows that supplementation of pooled HMOs in mice changed immune cell composition in the spleen and mesenteric lymph nodes and immunoglobulins in circulation. Hence, this study highlights that maternal secretor status has a role in infant gut microbiota composition, and this, in turn, can impact host gut and immune system.
Competing Interests: The authors declare no conflict of interest.
Importance: Early life factors like neonatal diet modulate gut microbiota, which is important for the optimal gut and immune function. One such factor, human milk oligosaccharides (HMOs), the composition of which is determined by maternal secretor status, has a profound effect on infant gut microbiota. However, how the infant gut microbiota composition determined by maternal secretor status or consumption of infant formula devoid of HMOs impacts infant intestinal ammorphology, gene expression, and immune signature is not well explored. This study provides insights into the differential establishment of infant microbiota derived from infants fed by secretor or non-secretor mothers milk or those consuming infant formula and demonstrates that the secretor status of mothers promotes Bifidobacteria and Bacteroides sps. establishment. This study also shows that supplementation of pooled HMOs in mice changed immune cell composition in the spleen and mesenteric lymph nodes and immunoglobulins in circulation. Hence, this study highlights that maternal secretor status has a role in infant gut microbiota composition, and this, in turn, can impact host gut and immune system.
Competing Interests: The authors declare no conflict of interest.