학술논문
Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve.
Document Type
Academic Journal
Author
Telfer AC; Biodiversity Institute of Ontario, Guelph, Canada.; Young MR; Biodiversity Institute of Ontario, Guelph, Canada.; Quinn J; rare Charitable Research Reserve, Cambridge, Canada.; Perez K; Biodiversity Institute of Ontario, Guelph, Canada.; Sobel CN; Biodiversity Institute of Ontario, Guelph, Canada.; Sones JE; Biodiversity Institute of Ontario, Guelph, Canada.; Levesque-Beaudin V; Biodiversity Institute of Ontario, Guelph, Canada.; Derbyshire R; Biodiversity Institute of Ontario, Guelph, Canada.; Fernandez-Triana J; CNC, Ottawa, Canada.; Rougerie R; Muséum national d'Histoire Naturelle, Paris, France.; Thevanayagam A; Biodiversity Institute of Ontario, Guelph, Canada.; Boskovic A; Biodiversity Institute of Ontario, Guelph, Canada.; Borisenko AV; Biodiversity Institute of Ontario, Guelph, Canada.; Cadel A; University of Waterloo, Waterloo, Canada.; Brown A; Biodiversity Institute of Ontario, Guelph, Canada.; Pages A; Université de Montpellier, Montpellier, France.; Castillo AH; Biodiversity Institute of Ontario, Guelph, Canada.; Nicolai A; EcoBio, Université of Rennes, Rennes, France.; Glenn Mockford BM; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Bukowski B; Museo Argentino de Ciencias Naturales 'Bernardino Rivadavia' (MACN-CONICET), Buenos Aires, Argentina.; Wilson B; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Trojahn B; rare Charitable Research Reserve, Cambridge, Canada.; Lacroix CA; Biodiversity Institute of Ontario Herbarium, Guelph, Canada.; Brimblecombe C; University of Waikato, Hamilton, New Zealand.; Hay C; University of Western Ontario, London, Canada.; Ho C; Biodiversity Institute of Ontario, Guelph, Canada.; Steinke C; Biodiversity Institute of Ontario, Guelph, Canada.; Warne CP; Biodiversity Institute of Ontario, Guelph, Canada.; Garrido Cortes C; University of Guelph, Guelph, Canada.; Engelking D; Biodiversity Institute of Ontario, Guelph, Canada.; Wright D; Biodiversity Institute of Ontario, Guelph, Canada.; Lijtmaer DA; Museo Argentino de Ciencias Naturales 'Bernardino Rivadavia' (MACN-CONICET), Buenos Aires, Argentina.; Gascoigne D; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Hernandez Martich D; Universidad Autonoma de Santo Domingo DR, Santo Domingo, Dominican Republic.; Morningstar D; Myotistar, Cambridge, Canada.; Neumann D; SNSB, Zoologische Staatssammlung Muenchen, Munich, Germany.; Steinke D; Biodiversity Institute of Ontario, Guelph, Canada.; Marco DeBruin DD; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Dobias D; University of Guelph, Guelph, Canada.; Sears E; Biodiversity Institute of Ontario, Guelph, Canada.; Richard E; University of Guelph, Guelph, Canada.; Damstra E; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Zakharov EV; Biodiversity Institute of Ontario, Guelph, Canada.; Laberge F; University of Guelph, Guelph, Canada.; Collins GE; University of Waikato, Hamilton, New Zealand.; Blagoev GA; Biodiversity Institute of Ontario, Guelph, Canada.; Grainge G; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Ansell G; Biodiversity Institute of Ontario, Guelph, Canada.; Meredith G; Grand River Conservation Authority, Guelph, Canada.; Hogg I; University of Waikato, Hamilton, New Zealand.; McKeown J; Biodiversity Institute of Ontario, Guelph, Canada.; Topan J; Biodiversity Institute of Ontario, Guelph, Canada.; Bracey J; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Guenther J; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Sills-Gilligan J; Biodiversity Institute of Ontario, Guelph, Canada.; Addesi J; Biodiversity Institute of Ontario, Guelph, Canada.; Persi J; Biodiversity Institute of Ontario, Guelph, Canada.; Layton KK; The University of Western Australia, Perth, Australia.; D'Souza K; Biodiversity Institute of Ontario, Guelph, Canada.; Dorji K; National Biodiversity Centre, Thimphu, Bhutan.; Grundy K; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Nghidinwa K; Ministry of Environment and Tourism in Namibia, Windhoek, Namibia.; Ronnenberg K; Biodiversity Institute of Ontario, Guelph, Canada.; Lee KM; University of Oulu, Oulu, Finland.; Xie L; The University of Western Ontario, London, Canada.; Lu L; Biodiversity Institute of Ontario, Guelph, Canada.; Penev L; Pensoft, Sofia, Bulgaria.; Gonzalez M; Instituto de Investigación de Recursos Biológicos Alexander von Humboldt, Bogotá, Colombia.; Rosati ME; Smithsonian National Museum of Natural History, Washington, United States of America.; Kekkonen M; Biodiversity Institute of Ontario, Guelph, Canada.; Kuzmina M; Biodiversity Institute of Ontario, Guelph, Canada.; Iskandar M; Biodiversity Institute of Ontario, Guelph, Canada.; Mutanen M; University of Oulu, Oulu, Finland.; Fatahi M; Biodiversity Institute of Ontario, Guelph, Canada.; Pentinsaari M; University of Oulu, Oulu, Finland.; Bauman M; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Nikolova N; Biodiversity Institute of Ontario, Guelph, Canada.; Ivanova NV; Biodiversity Institute of Ontario, Guelph, Canada.; Jones N; Biodiversity Institute of Ontario, Guelph, Canada.; Weerasuriya N; The University of Western Ontario, London, Canada.; Monkhouse N; Biodiversity Institute of Ontario, Guelph, Canada.; Lavinia PD; Museo Argentino de Ciencias Naturales 'Bernardino Rivadavia' (MACN-CONICET), Buenos Aires, Argentina.; Jannetta P; Biodiversity Institute of Ontario, Guelph, Canada.; Hanisch PE; Museo Argentino de Ciencias Naturales 'Bernardino Rivadavia' (MACN-CONICET), Buenos Aires, Argentina.; McMullin RT; Biodiversity Institute of Ontario Herbarium, Guelph, Canada.; Ojeda Flores R; Universidad Nacional Autónoma de México, Mexico City, Mexico.; Mouttet R; ANSES, Laboratoire de la Santé des Végétaux, Montferrier sur Lez, France.; Vender R; Biodiversity Institute of Ontario, Guelph, Canada.; Labbee RN; Biodiversity Institute of Ontario, Guelph, Canada.; Forsyth R; New Brunswick Museum, Saint John, Canada.; Lauder R; London Homeopathy, London, Canada.; Dickson R; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Kroft R; rare Charitable Research Reserve (Affiliate of), Cambridge, Canada.; Miller SE; Smithsonian National Museum of Natural History, Washington, United States of America.; MacDonald S; Biodiversity Institute of Ontario, Guelph, Canada.; Panthi S; Ministry of Forests and Soil Conservation, Kathmandu, Nepal.; Pedersen S; Biodiversity Institute of Ontario, Guelph, Canada.; Sobek-Swant S; rare Charitable Research Reserve, Cambridge, Canada.; Naik S; Biodiversity Institute of Ontario, Guelph, Canada.; Lipinskaya T; Scientific and Practical Center for Bioresources, National Academy of Sciences of Belarus, Minsk, Belarus.; Eagalle T; Biodiversity Institute of Ontario, Guelph, Canada.; Decaëns T; Université de Montpellier Centre d'Ecologie Fonctionnelle et Evolutive, Montpellier, France.; Kosuth T; Université de Montpellier, Montpellier, France.; Braukmann T; Biodiversity Institute of Ontario, Guelph, Canada.; Woodcock T; rare Charitable Research Reserve, Cambridge, Canada.; Roslin T; University of Helsinki, Helsinki, Finland ; Swedish University of Agricultural Sciences, Uppsala, Sweden.; Zammit T; Grand River Conservation Authority, Cambridge, Canada.; Campbell V; Biodiversity Institute of Ontario, Guelph, Canada.; Dinca V; Biodiversity Institute of Ontario, Guelph, Canada.; Peneva V; Bulgarian Academy of Sciences, Sofia, Bulgaria.; Hebert PD; Biodiversity Institute of Ontario, Guelph, Canada.; deWaard JR; Biodiversity Institute of Ontario, Guelph, Canada.
Source
Publisher: Pensoft Publishers Country of Publication: Bulgaria NLM ID: 101619899 Publication Model: eCollection Cited Medium: Print ISSN: 1314-2828 (Print) Linking ISSN: 13142828 NLM ISO Abbreviation: Biodivers Data J Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
1314-2828
Abstract
Background: Comprehensive biotic surveys, or 'all taxon biodiversity inventories' (ATBI), have traditionally been limited in scale or scope due to the complications surrounding specimen sorting and species identification. To circumvent these issues, several ATBI projects have successfully integrated DNA barcoding into their identification procedures and witnessed acceleration in their surveys and subsequent increase in project scope and scale. The Biodiversity Institute of Ontario partnered with the rare Charitable Research Reserve and delegates of the 6th International Barcode of Life Conference to complete its own rapid, barcode-assisted ATBI of an established land trust in Cambridge, Ontario, Canada.
New Information: The existing species inventory for the rare Charitable Research Reserve was rapidly expanded by integrating a DNA barcoding workflow with two surveying strategies - a comprehensive sampling scheme over four months, followed by a one-day bioblitz involving international taxonomic experts. The two surveys resulted in 25,287 and 3,502 specimens barcoded, respectively, as well as 127 human observations. This barcoded material, all vouchered at the Biodiversity Institute of Ontario collection, covers 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi, and lichens. Overall, the ATBI documented 1,102 new species records for the nature reserve, expanding the existing long-term inventory by 49%. In addition, 2,793 distinct Barcode Index Numbers (BINs) were assigned to genus or higher level taxonomy, and represent additional species that will be added once their taxonomy is resolved. For the 3,502 specimens, the collection, sequence analysis, taxonomic assignment, data release and manuscript submission by 100+ co-authors all occurred in less than one week. This demonstrates the speed at which barcode-assisted inventories can be completed and the utility that barcoding provides in minimizing and guiding valuable taxonomic specialist time. The final product is more than a comprehensive biotic inventory - it is also a rich dataset of fine-scale occurrence and sequence data, all archived and cross-linked in the major biodiversity data repositories. This model of rapid generation and dissemination of essential biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially be employed for evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011-2020.
New Information: The existing species inventory for the rare Charitable Research Reserve was rapidly expanded by integrating a DNA barcoding workflow with two surveying strategies - a comprehensive sampling scheme over four months, followed by a one-day bioblitz involving international taxonomic experts. The two surveys resulted in 25,287 and 3,502 specimens barcoded, respectively, as well as 127 human observations. This barcoded material, all vouchered at the Biodiversity Institute of Ontario collection, covers 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi, and lichens. Overall, the ATBI documented 1,102 new species records for the nature reserve, expanding the existing long-term inventory by 49%. In addition, 2,793 distinct Barcode Index Numbers (BINs) were assigned to genus or higher level taxonomy, and represent additional species that will be added once their taxonomy is resolved. For the 3,502 specimens, the collection, sequence analysis, taxonomic assignment, data release and manuscript submission by 100+ co-authors all occurred in less than one week. This demonstrates the speed at which barcode-assisted inventories can be completed and the utility that barcoding provides in minimizing and guiding valuable taxonomic specialist time. The final product is more than a comprehensive biotic inventory - it is also a rich dataset of fine-scale occurrence and sequence data, all archived and cross-linked in the major biodiversity data repositories. This model of rapid generation and dissemination of essential biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially be employed for evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011-2020.