학술논문
Bifidobacterium infantis associates with T cell immunity in human infants and is sufficient to enhance antigen-specific T cells in mice.
Document Type
Academic Journal
Author
Nyangahu DD; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Happel AU; Institute of Infectious Diseases and Molecular Medicine, Department of Pathology, Division of Immunology, University of Cape Town, Cape Town, South Africa.; Wendoh J; Institute of Infectious Diseases and Molecular Medicine, Department of Pathology, Division of Immunology, University of Cape Town, Cape Town, South Africa.; Kiravu A; Institute of Infectious Diseases and Molecular Medicine, Department of Pathology, Division of Immunology, University of Cape Town, Cape Town, South Africa.; Wang Y; Department of Bioengineering, University of Washington, Seattle, WA, USA.; Feng C; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Plumlee C; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Cohen S; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Brown BP; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Djukovic D; Northwest Metabolomics Research Center, University of Washington, Seattle, WA, USA.; Ganief T; Institute of Infectious Diseases and Molecular Medicine, Department of Integrative Biomedical Sciences, Division of Chemical and Systems Biology, University of Cape Town, Cape Town, South Africa.; Gasper M; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Raftery D; Northwest Metabolomics Research Center, University of Washington, Seattle, WA, USA.; Blackburn JM; Institute of Infectious Diseases and Molecular Medicine, Department of Integrative Biomedical Sciences, Division of Chemical and Systems Biology, University of Cape Town, Cape Town, South Africa.; Allbritton NL; Department of Bioengineering, University of Washington, Seattle, WA, USA.; Gray CM; Institute of Infectious Diseases and Molecular Medicine, Department of Pathology, Division of Immunology, University of Cape Town, Cape Town, South Africa.; Biomedical Research Institute, Division of Molecular Biology and Human Genetics, Stellenbosch University, Cape Town, South Africa.; Paik J; Department of Comparative Medicine, University of Washington, Seattle, WA, USA.; Urdahl KB; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Department of Pediatrics, School of Medicine, University of Washington, Seattle WA, USA.; Jaspan HB; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.; Institute of Infectious Diseases and Molecular Medicine, Department of Pathology, Division of Immunology, University of Cape Town, Cape Town, South Africa.; Department of Pediatrics, School of Medicine, University of Washington, Seattle WA, USA.
Source
Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE
Subject
Language
English
Abstract
Bacille Calmette-Guerin (BCG) vaccine can elicit good T H 1 responses in neonates. We hypothesized that the pioneer gut microbiota affects vaccine T cell responses. Infants who are HIV exposed but uninfected (iHEU) display an altered immunity to vaccination. BCG-specific immune responses were analyzed at 7 weeks of age in iHEU, and responses were categorized as high or low. Bifidobacterium longum subsp. infantis was enriched in the stools of high responders, while Bacteroides thetaiotaomicron was enriched in low responders at time of BCG vaccination. Neonatal germ-free or SPF mice orally gavaged with live B. infantis exhibited significantly higher BCG-specific T cells compared with pups gavaged with B. thetaiotaomicron. B. infantis and B. thetaiotaomicron differentially affected stool metabolome and colonic transcriptome. Human colonic epithelial cells stimulated with B. infantis induced a unique gene expression profile versus B. thetaiotaomicron . We thus identified a causal role of B. infantis in early-life antigen-specific immunity.