학술논문
TNF ΔARE Pigs: A Translational Crohn's Disease Model.
Document Type
Academic Journal
Author
Winogrodzki T; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Metwaly A; Chair of Nutrition and Immunology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Grodziecki A; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Liang W; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Klinger B; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Flisikowska T; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Fischer K; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Flisikowski K; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Steiger K; Comparative Experimental Pathology, Institute of Pathology, Technical University of Munich, Munich, Germany.; Haller D; Chair of Nutrition and Immunology, School of Life Sciences, Technical University of Munich, Freising, Germany.; Schnieke A; Chair of Livestock Biotechnology, School of Life Sciences, Technical University of Munich, Freising, Germany.
Source
Publisher: Oxford University Press Country of Publication: England NLM ID: 101318676 Publication Model: Print Cited Medium: Internet ISSN: 1876-4479 (Electronic) Linking ISSN: 18739946 NLM ISO Abbreviation: J Crohns Colitis Subsets: MEDLINE
Subject
Language
English
Abstract
Background and Aims: Crohn's disease [CD] is a major subtype of inflammatory bowel diseases [IBD] with increasing incidence and prevalence. Results of studies using available small and large animal models are often poorly translatable to patients, and few CD models show small intestinal pathology. Due to its similarities to humans, the pig has emerged as a highly suitable translational disease model, particularly for testing novel nutritional and technological interventions. Our goal was to develop a physiologically relevant porcine CD model to facilitate translation of findings and interventions towards the clinic.
Methods: We generated pigs bearing a 93-bp deletion of the adenosine-uracil-rich element [ARE] and a constitutive-decay element within the 3' untranslated region of the TNF gene. Comparative analysis of physiological, molecular, histological and microbial characteristics was performed between wild-type, TNFΔARE/+ and TNFΔARE/ΔARE animals. Alterations in the microbiome were compared to the TNFΔARE mouse model and IBD patients.
Results: TNF ΔARE pigs recapitulate major characteristics of human CD, including ulcerative transmural ileocolitis, increased abundance of proinflammatory cytokines, immune cell infiltration and dysbiotic microbial communities. 16S rRNA gene amplicon sequencing revealed enrichment in members belonging to Megasphaera, Campylobacter, Desulfovibrio, Alistipes and Lachnoclostridum in faecal or mucosa-associated bacteria compared to wild-type littermates. Principal components analysis clustering with a subset of TNFΔARE/+ mice and human IBD patients suggests microbial similarity based on disease severity.
Conclusions: We demonstrate that the TNFΔARE pig resembles a CD-like ileocolitis pathophenotype recapitulating human disease. The ability to conduct long-term studies and test novel surgical procedures and dietary interventions in a physiologically relevant model will benefit future translational IBD research studies.
(© The Author(s) 2023. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation.)
Methods: We generated pigs bearing a 93-bp deletion of the adenosine-uracil-rich element [ARE] and a constitutive-decay element within the 3' untranslated region of the TNF gene. Comparative analysis of physiological, molecular, histological and microbial characteristics was performed between wild-type, TNFΔARE/+ and TNFΔARE/ΔARE animals. Alterations in the microbiome were compared to the TNFΔARE mouse model and IBD patients.
Results: TNF ΔARE pigs recapitulate major characteristics of human CD, including ulcerative transmural ileocolitis, increased abundance of proinflammatory cytokines, immune cell infiltration and dysbiotic microbial communities. 16S rRNA gene amplicon sequencing revealed enrichment in members belonging to Megasphaera, Campylobacter, Desulfovibrio, Alistipes and Lachnoclostridum in faecal or mucosa-associated bacteria compared to wild-type littermates. Principal components analysis clustering with a subset of TNFΔARE/+ mice and human IBD patients suggests microbial similarity based on disease severity.
Conclusions: We demonstrate that the TNFΔARE pig resembles a CD-like ileocolitis pathophenotype recapitulating human disease. The ability to conduct long-term studies and test novel surgical procedures and dietary interventions in a physiologically relevant model will benefit future translational IBD research studies.
(© The Author(s) 2023. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation.)