학술논문
Intracellular Staphylococcus aureus Elicits the Production of Host Very Long-Chain Saturated Fatty Acids with Antimicrobial Activity.
Document Type
Academic Journal
Author
Bravo-Santano N; Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UK.; Ellis JK; Division of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London W12 0HS, UK.; Calle Y; Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UK.; Keun HC; Division of Cancer, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London W12 0HS, UK.; Behrends V; Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UK. Volker.Behrends@roehampton.ac.uk.; Letek M; Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UK. Michal.Letek@roehampton.ac.uk.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101578790 Publication Model: Electronic Cited Medium: Print ISSN: 2218-1989 (Print) Linking ISSN: 22181989 NLM ISO Abbreviation: Metabolites Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2218-1989
Abstract
As a facultative intracellular pathogen, Staphylococcus aureus is able to invade and proliferate within many types of mammalian cells. Intracellular bacterial replication relies on host nutrient supplies and, therefore, cell metabolism is closely bound to intracellular infection. Here, we investigated how S. aureus invasion affects the host membrane-bound fatty acids. We quantified the relative levels of fatty acids and their labelling pattern after intracellular infection by gas chromatography-mass spectrometry (GC-MS). Interestingly, we observed that the levels of three host fatty acids-docosanoic, eicosanoic and palmitic acids-were significantly increased in response to intracellular S. aureus infection. Accordingly, labelling carbon distribution was also affected in infected cells, in comparison to the uninfected control. In addition, treatment of HeLa cells with these three fatty acids showed a cytoprotective role by directly reducing S. aureus growth.