학술논문
Chick heterophils release DNA extracellular traps (DETs) in vitro and in vivo upon Aspergillus fumigatus conidia exposure.
Document Type
Academic Journal
Author
Lima-Gomes PS; Instituto de Microbiologia Paulo de Góes, Departamento de Imunologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil; Instituto de Veterinária, Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil.; do Nascimento MTC; Instituto de Microbiologia Paulo de Góes, Departamento de Imunologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.; Nadaes NR; Instituto de Microbiologia Paulo de Góes, Departamento de Imunologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.; de Campos SG; Instituto de Veterinária, Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil.; Tavares Haido RM; Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Rio de Janeiro, RJ, Brazil.; Danelli MDG; Instituto de Veterinária, Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil.; Pinto-da-Silva LH; Instituto de Veterinária, Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil.; Saraiva EM; Instituto de Microbiologia Paulo de Góes, Departamento de Imunologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Electronic address: esaraiva@micro.ufrj.br.
Source
Publisher: Elsevier Country of Publication: France NLM ID: 100883508 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1769-714X (Electronic) Linking ISSN: 12864579 NLM ISO Abbreviation: Microbes Infect Subsets: MEDLINE
Subject
Language
English
Abstract
Aspergillosis is a common fungal disease in avian species, causing high mortality in young chicks in agricultural farms and yards. It is caused by fungi belonging to the genus Aspergillus. Aspergillosis occurs by inhalation of fungal conidia, and in chickens, effective infection control relies on a rapid and large influx of heterophils to the lungs. Heterophils, upon different stimuli, release to the extracellular milieu their chromatin associated with several proteins that ensnare and kill different pathogens similarly to neutrophil extracellular traps. Here, we showed that Aspergillus fumigatus conidia and the peptidogalactomannan (PGM), isolated from the fungus cell wall, induce the release of DNA extracellular traps (DETs) in chicks' blood and lung heterophils. We demonstrated that reactive oxygen species, elastase and peptidyl arginine deiminase (PAD) were involved in DETs extrusion, the occurrence of DETs in the lungs of A. fumigatus-exposed chicks in vivo, and its role in chick survival. These results may contribute to developing more efficient tools for the therapeutic and diagnosis of aspergillosis.
Competing Interests: Declaration of competing interest The authors have declared that no competing interest exists.
(Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Competing Interests: Declaration of competing interest The authors have declared that no competing interest exists.
(Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)