학술논문
Comparative evaluation of three histidine-rich Protein-2 based rapid diagnostic tests, microscopy and PCR for guiding malaria treatment in Ibadan, Southwest Nigeria.
Document Type
Academic Journal
Author
Orimadegun AE; Institute of Child Health, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Funwei RI; Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan; Department of Pharmacy Technician Studies, Bayelsa State College of Health Technology, Ibadan, Nigeria.; Michael OS; Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Ogunkunle OO; Department of Paediatrics, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Badejo JA; Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Olusola FI; Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Agede O; Department of Pharmacology and Therapeutics, University of Ilorin, Ilorin, Nigeria.; Anjorin OE; Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Ajayi IO; Department of Epidemiology and Medical Statistics, College of Medicine, University of Ibadan, Ibadan, Nigeria.; Jegede AS; Department of Sociology, Faculty of The Social Sciences, University of Ibadan, Ibadan, Nigeria.; Ojurongbe O; Department of Medical Microbiology and Parasitology, Ladoke Akintola University of Technology, Osogbo, Nigeria.; Falade CO; Department of Pharmacology and Therapeutics; Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Source
Publisher: Medknow Publications Country of Publication: India NLM ID: 101150032 Publication Model: Print Cited Medium: Internet ISSN: 1119-3077 (Print) NLM ISO Abbreviation: Niger J Clin Pract Subsets: MEDLINE
Subject
Language
English
ISSN
1119-3077
Abstract
Background: Malaria rapid diagnostic tests (mRDTs) are the preferred option for programmatic deployment.
Aims: There are numerous mRDTs on the Nigerian market and there is a need to guide practitioners on the relative performance of the commonly used brands of mRDT in Nigeria.
Subjects and Methods: The performance of three commonly used Histidine-Rich-Protein-2-based mRDTs (SD-Bioline™, Carestart™ and Paracheck-Pf™) against microscopy of Giemsa stained blood and polymerase chain reaction (PCR) was evaluated among 190 febrile under-5 children in Ibadan, Nigeria. We calculated the sensitivity, specificity, predictive values, accuracy, and agreements.
Results: There were 53.2% males. The prevalence of malaria parasite by microscopy was 46.8% and 57.9% by PCR. Malaria parasite detection by SD-Bioline™ was 60.5%, Carestart™: 60.0% and Paracheck-Pf™ 60.0%. Using microscopy as the gold standard, the sensitivities of SD-Bioline™, Carestart™ and Paracheck-Pf™ mRDT were 97.8%, 96.7% and 97.8% respectively while the specificities were 73.0%, 72.0% and 74.0% respectively. Using PCR as the gold standard, the sensitivity for both SD-Bioline™ and Paracheck-Pf™ was 85.5% and for CareStart was 84.6% while the specificity of SD-Bioline™, Carestart™, and Paracheck-Pf™ was 73.8%, 72.4%, and 75.0% respectively. The test accuracy was 81.0% for both SD-Bioline™ and Paracheck-Pf™ and 80.0% for Caresatrt™. The kappa coefficient of agreement between PCR and each of SD-Bioline™, Carestart, ParaCheck™ and microscopy was 0.597, 0.578, 0.609 and 0.739 respectively.
Conclusion: The performance of the three mRDTs is a proof that any of the three is suitable for use in the diagnosis of malaria in the southwest of Nigeria.
Competing Interests: None
Aims: There are numerous mRDTs on the Nigerian market and there is a need to guide practitioners on the relative performance of the commonly used brands of mRDT in Nigeria.
Subjects and Methods: The performance of three commonly used Histidine-Rich-Protein-2-based mRDTs (SD-Bioline™, Carestart™ and Paracheck-Pf™) against microscopy of Giemsa stained blood and polymerase chain reaction (PCR) was evaluated among 190 febrile under-5 children in Ibadan, Nigeria. We calculated the sensitivity, specificity, predictive values, accuracy, and agreements.
Results: There were 53.2% males. The prevalence of malaria parasite by microscopy was 46.8% and 57.9% by PCR. Malaria parasite detection by SD-Bioline™ was 60.5%, Carestart™: 60.0% and Paracheck-Pf™ 60.0%. Using microscopy as the gold standard, the sensitivities of SD-Bioline™, Carestart™ and Paracheck-Pf™ mRDT were 97.8%, 96.7% and 97.8% respectively while the specificities were 73.0%, 72.0% and 74.0% respectively. Using PCR as the gold standard, the sensitivity for both SD-Bioline™ and Paracheck-Pf™ was 85.5% and for CareStart was 84.6% while the specificity of SD-Bioline™, Carestart™, and Paracheck-Pf™ was 73.8%, 72.4%, and 75.0% respectively. The test accuracy was 81.0% for both SD-Bioline™ and Paracheck-Pf™ and 80.0% for Caresatrt™. The kappa coefficient of agreement between PCR and each of SD-Bioline™, Carestart, ParaCheck™ and microscopy was 0.597, 0.578, 0.609 and 0.739 respectively.
Conclusion: The performance of the three mRDTs is a proof that any of the three is suitable for use in the diagnosis of malaria in the southwest of Nigeria.
Competing Interests: None