학술논문
Impact of SARS-CoV-2 on saliva: TNF-⍺, IL-6, IL-10, lactoferrin, lysozyme, IgG, IgA, and IgM.
Document Type
Academic Journal
Author
Santos JGO; ENT Research Lab, Department of Otorhinolaryngology-Head and Neck Surgery, Federal University of São Paulo, São Paulo, SP, Brazil; IPO Hospital, Paranaense Institute of Otorhinolaryngology, Paraná, Brazil.; Migueis DP; Antonio Pedro University Hospital, Fluminense Federal University, Niterói, Brazil.; Amaral JBD; ENT Research Lab, Department of Otorhinolaryngology-Head and Neck Surgery, Federal University of São Paulo, São Paulo, SP, Brazil.; Bachi ALL; ENT Research Lab, Department of Otorhinolaryngology-Head and Neck Surgery, Federal University of São Paulo, São Paulo, SP, Brazil.; Boggi AC; ENT Research Lab, Department of Otorhinolaryngology-Head and Neck Surgery, Federal University of São Paulo, São Paulo, SP, Brazil; Corpo de Bombeiros da Policia Militar do Estado de São Paulo, Brazil.; Thamboo A; Department of Surgery, University of British Columbia, Vancouver, Canada.; Voegels RL; Department of Ophthalmology and Otorhinolaryngology, University of São Paulo, São Paulo, SP, Brazil.; Pezato R; ENT Research Lab, Department of Otorhinolaryngology-Head and Neck Surgery, Federal University of São Paulo, São Paulo, SP, Brazil; Department of Ophthalmology and Otorhinolaryngology, University of São Paulo, São Paulo, SP, Brazil. Electronic address: pezatobau@gmail.com.
Source
Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101226721 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1880-3865 (Electronic) Linking ISSN: 13490079 NLM ISO Abbreviation: J Oral Biosci Subsets: MEDLINE
Subject
Language
English
Abstract
Objectives: Understanding the role of certain salivary components, such as TNF-⍺, IL-6, IL-10, lactoferrin, lysozyme, IgG, IgA, and IgM, in airway defense during the ongoing SARS-CoV-2 pandemic is essential. The salivary immune barrier of patients with COVID-19 may play a role in their prognosis. The present study aims to evaluate the impact of SARS-CoV-2 on saliva composition.
Methods: A longitudinal study was carried out with male and female firefighters aged 24-48 years. The study sample (n = 34) was divided into 3 groups: asymptomatic volunteers with a negative polymerase chain reaction (PCR) test for SARS-CoV-2 (group 1, Control, n = 21); patients with symptoms of COVID-19 of less than 7 days' duration and a diagnosis of SARS-CoV-2 infection by PCR (group 2, COVID-19, n = 13); and recovered patients from group 2 who were free of COVID-19 symptoms for at least 2 months (group 3, post-COVID-19 recovery, n = 13). All groups underwent real-time PCR to detect the presence of SARS-CoV-2, as well as analysis of the salivary concentrations of TNF-⍺, IL-6, IL-10, lactoferrin, lysozyme, IgG, IgA, and IgM by the ELISA method.
Results: Lactoferrin concentrations were significantly decreased in the infected group (COVID-19) when compared to those not infected by SARS-CoV-2 (control) (p = 0.032). IgA concentrations were decreased in the COVID-19 and post-COVID-19 groups compared to the control group (p = 0.005 and p = 0.016, respectively). Comparison of the COVID-19 and post-COVID-19 groups also revealed an increase in IgM concentrations during acute SARS-CoV-2 infection (p = 0.010).
Conclusion: SARS-CoV-2 alters the composition of the salivary immune barrier.
Competing Interests: Conflict of interest No potential conflicts of interest are disclosed.
(Copyright © 2022 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.)
Methods: A longitudinal study was carried out with male and female firefighters aged 24-48 years. The study sample (n = 34) was divided into 3 groups: asymptomatic volunteers with a negative polymerase chain reaction (PCR) test for SARS-CoV-2 (group 1, Control, n = 21); patients with symptoms of COVID-19 of less than 7 days' duration and a diagnosis of SARS-CoV-2 infection by PCR (group 2, COVID-19, n = 13); and recovered patients from group 2 who were free of COVID-19 symptoms for at least 2 months (group 3, post-COVID-19 recovery, n = 13). All groups underwent real-time PCR to detect the presence of SARS-CoV-2, as well as analysis of the salivary concentrations of TNF-⍺, IL-6, IL-10, lactoferrin, lysozyme, IgG, IgA, and IgM by the ELISA method.
Results: Lactoferrin concentrations were significantly decreased in the infected group (COVID-19) when compared to those not infected by SARS-CoV-2 (control) (p = 0.032). IgA concentrations were decreased in the COVID-19 and post-COVID-19 groups compared to the control group (p = 0.005 and p = 0.016, respectively). Comparison of the COVID-19 and post-COVID-19 groups also revealed an increase in IgM concentrations during acute SARS-CoV-2 infection (p = 0.010).
Conclusion: SARS-CoV-2 alters the composition of the salivary immune barrier.
Competing Interests: Conflict of interest No potential conflicts of interest are disclosed.
(Copyright © 2022 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.)