학술논문
Association between single-nucleotide polymorphisms in serotonin receptor 2A and melatonin receptor 1A genes and pain after root canal treatment.
Document Type
Academic Journal
Author
Meyfarth SRS; Postgraduate Program, School of Dentistry, Fluminense Federal University, Niterói, Brazil.; Tavares JDS; Clinical Research Unit, Fluminense Federal University, Rio de Janeiro, Brazil.; Guimarães LDS; Postgraduate Program, School of Dentistry, Fluminense Federal University, Niterói, Brazil.; Silva EAB; Postgraduate Program, School of Dentistry, Fluminense Federal University, Nova Friburgo, Brazil.; Gaio DC; School of Health and Biological Sciences, Positivo University, Curitiba, Brazil.; Ecker MB; School of Health and Biological Sciences, Positivo University, Curitiba, Brazil.; Brancher JA; School of Health and Biological Sciences, Positivo University, Curitiba, Brazil.; Küchler EC; University of Tuiuti, Curitiba, Brazil.; Silva-Sousa AC; Restorative Dentistry Department, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.; de Sousa-Neto MD; Restorative Dentistry Department, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.; Antunes LAA; Postgraduate Program, School of Dentistry, Fluminense Federal University, Niterói, Brazil.; Clinical Research Unit, Fluminense Federal University, Rio de Janeiro, Brazil.; Postgraduate Program, School of Dentistry, Fluminense Federal University, Nova Friburgo, Brazil.; Specific Formation Department, School of Dentistry of Nova Friburgo, Fluminense Federal University, Rio de Janeiro, Brazil.; Antunes LS; Postgraduate Program, School of Dentistry, Fluminense Federal University, Niterói, Brazil.; Clinical Research Unit, Fluminense Federal University, Rio de Janeiro, Brazil.; Postgraduate Program, School of Dentistry, Fluminense Federal University, Nova Friburgo, Brazil.; Specific Formation Department, School of Dentistry of Nova Friburgo, Fluminense Federal University, Rio de Janeiro, Brazil.
Source
Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8004996 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2591 (Electronic) Linking ISSN: 01432885 NLM ISO Abbreviation: Int Endod J
Subject
Language
English
Abstract
Aim: This study aimed to investigate whether single-nucleotide polymorphisms (SNPs) in the genes encoding 5-HTR2A (5-Hydroxytryptamine (serotonin) receptor 2A) and MTNR1A (melatonin receptor 1A) may contribute to postoperative pain perception after root canal treatment. We hypothesised that SNPs in HTR2A and MTNR1A genes were associated with postoperative pain after root canal treatment.
Methodology: This genetic cohort study enrolled patients with single-rooted teeth diagnosed with pulp necrosis and asymptomatic apical periodontitis before root canal treatment. Root canal treatment was performed in one session using a standardized protocol. Postoperative pain and tenderness were assessed using a visual analogue scale (recorded every day for 7 days and on the 14th and 30th days after root canal treatment). Genomic DNA was extracted from saliva and used to genotype the SNPs in HTR2A (rs4941573 and rs6313) and MTNR1A (rs6553010, rs6847693 and rs13140012) using real-time polymerase chain reaction. Genotypes were compared using univariate and multivariate Poisson regression with generalized estimating equations (p < .05).
Results: In total, 108 patients were enrolled in this study. The SNPs rs6553010 (MTNR1A), rs4941573 and rs6313 (HTR2A) were associated with an increased risk of developing pain after root canal treatment (p < .05).
Conclusions: This study suggests that SNPs in HTR2A and MTNR1A influence pain response after root canal treatment.
(© 2023 British Endodontic Society. Published by John Wiley & Sons Ltd.)
Methodology: This genetic cohort study enrolled patients with single-rooted teeth diagnosed with pulp necrosis and asymptomatic apical periodontitis before root canal treatment. Root canal treatment was performed in one session using a standardized protocol. Postoperative pain and tenderness were assessed using a visual analogue scale (recorded every day for 7 days and on the 14th and 30th days after root canal treatment). Genomic DNA was extracted from saliva and used to genotype the SNPs in HTR2A (rs4941573 and rs6313) and MTNR1A (rs6553010, rs6847693 and rs13140012) using real-time polymerase chain reaction. Genotypes were compared using univariate and multivariate Poisson regression with generalized estimating equations (p < .05).
Results: In total, 108 patients were enrolled in this study. The SNPs rs6553010 (MTNR1A), rs4941573 and rs6313 (HTR2A) were associated with an increased risk of developing pain after root canal treatment (p < .05).
Conclusions: This study suggests that SNPs in HTR2A and MTNR1A influence pain response after root canal treatment.
(© 2023 British Endodontic Society. Published by John Wiley & Sons Ltd.)