학술논문
The p-ERG spatial acuity in the biomedical pig under physiological conditions.
Document Type
Academic Journal
Author
Ventrella D; Department of Veterinary Medicine, Alma Mater Studiorum-University of Bologna, Ozzano dell'Emilia, BO, Italy.; Maya-Vetencourt JF; Department of Biology, University of Pisa, Pisa, PI, Italy.; Center for Synaptic Neuroscience and Technology, Italian Institute of Technology IIT, Genoa, GE, Italy.; Elmi A; Department of Veterinary Medicine, Alma Mater Studiorum-University of Bologna, Ozzano dell'Emilia, BO, Italy. alberto.elmi2@unibo.it.; Barone F; Unit on Ocular Stem Cell and Translational Research, National Eye Institute (NEI), Bethesda, MD, USA.; Aniballi C; Department of Veterinary Medicine, Alma Mater Studiorum-University of Bologna, Ozzano dell'Emilia, BO, Italy.; Muscatello LV; Department of Veterinary Medicine, Alma Mater Studiorum-University of Bologna, Ozzano dell'Emilia, BO, Italy.; Mete M; IRCCS Sacro Cuore Don Calabria Hospital, Negrar, VR, Italy.; Pertile G; IRCCS Sacro Cuore Don Calabria Hospital, Negrar, VR, Italy.; Benfenati F; Center for Synaptic Neuroscience and Technology, Italian Institute of Technology IIT, Genoa, GE, Italy.; IRCCS San Martino Hospital, Genoa, GE, Italy.; Bacci ML; Department of Veterinary Medicine, Alma Mater Studiorum-University of Bologna, Ozzano dell'Emilia, BO, Italy.
Source
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Subject
Language
English
Abstract
Pigs are becoming an important pre-clinical animal species for translational ophthalmology, due to similarities with humans in anatomical and physiological patterns. Different models of eye disorders have been proposed, and they are good candidates to assess biocompatibility/functionality of retinal prostheses. Electroretinography is a common tool allowing to gain information on retinal function, with several types of electroretinogram (ERG) been implemented including full field (ff-ERG), multifocal (mf-ERG) and pattern (p-ERG). p-ERG represents a valuable tool to monitor Retinal Ganglion Cells (RGCs) activity and can be used to calculate p-ERG spatial acuity. Unfortunately, scarce methodological data are available regarding recording/interpretation of p-ERG and retinal acuity in biomedical pigs yet enhancing knowledge regarding pig vision physiology will allow for more refined and responsible use of such species. Aim of this study was to record p-ERG in juvenile pigs to functionally assess visual acuity. Six female hybrid pigs underwent two p-ERG recording sessions at 16 and 19 weeks of age. Photopic ff-ERG were also recorded; optical coherence tomography (OCT) and histology were used to confirm retinal integrity. ff-ERG signals were repeatable within/across sessions. All p-ERG traces consistently displayed characterizing peaks, and the progressive decrease of amplitude in response to the increment of spatial frequency revealed the reliability of the method. Mean p-ERG spatial acuities were 5.7 ± 0.14 (16 weeks) and 6.2 ± 0.15 cpd (19 weeks). Overall, the p-ERG recordings described in the present work seem reliable and repeatable, and may represent an important tool when it comes to vision assessment in pigs.
(© 2022. The Author(s).)
(© 2022. The Author(s).)