학술논문
Subretinally injected semiconducting polymer nanoparticles rescue vision in a rat model of retinal dystrophy.
Document Type
Academic Journal
Author
Maya-Vetencourt JF; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Department of Biology, University of Pisa, Pisa, Italy.; Manfredi G; Centre for Nano Science and Technology, Istituto Italiano di Tecnologia, Milan, Italy.; Mete M; Ophthalmology Department, IRCCS Ospedale Sacro Cuore Don Calabria, Negrar, Italy.; Colombo E; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Bramini M; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; Department of Applied Physics, University of Granada, Granada, Spain.; Di Marco S; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Shmal D; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; Mantero G; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; Dipalo M; Plasmon Nanotechnologies, Istituto Italiano di Tecnologia, Genoa, Italy.; Rocchi A; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; DiFrancesco ML; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; Papaleo ED; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; Russo A; Ophthalmology Department, IRCCS Ospedale Sacro Cuore Don Calabria, Negrar, Italy.; Barsotti J; Centre for Nano Science and Technology, Istituto Italiano di Tecnologia, Milan, Italy.; Eleftheriou C; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy.; Departments of Ophthalmology and Neurology, Weil Medical College of Cornell University, White Plains, NY, USA.; Di Maria F; CNR Institute of Organic Synthesis and Photoreactivity (ISOF), Bologna, Italy.; Cossu V; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Department of Health Science, Nuclear Medicine, University of Genoa, Genoa, Italy.; Piazza F; ENT Department, ASST Mantova, Mantua, Italy.; Emionite L; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Ticconi F; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Department of Health Science, Nuclear Medicine, University of Genoa, Genoa, Italy.; Department of Oncohematology, Nuclear Medicine Unit, Faenza Hospital, Faenza, Italy.; Marini C; CNR Institute of Bioimages and Molecular Physiology, Milan (Genoa Section), Genoa, Italy.; Sambuceti G; IRCCS Ospedale Policlinico San Martino, Genoa, Italy.; Department of Health Science, Nuclear Medicine, University of Genoa, Genoa, Italy.; Pertile G; Ophthalmology Department, IRCCS Ospedale Sacro Cuore Don Calabria, Negrar, Italy.; Lanzani G; Centre for Nano Science and Technology, Istituto Italiano di Tecnologia, Milan, Italy. guglielmo.lanzani@iit.it.; Department of Physics, Politecnico di Milano, Milan, Italy. guglielmo.lanzani@iit.it.; Benfenati F; Centre for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genoa, Italy. fabio.benfenati@iit.it.; IRCCS Ospedale Policlinico San Martino, Genoa, Italy. fabio.benfenati@iit.it.
Source
Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101283273 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1748-3395 (Electronic) Linking ISSN: 17483387 NLM ISO Abbreviation: Nat Nanotechnol Subsets: MEDLINE
Subject
Language
English
Abstract
Inherited retinal dystrophies and late-stage age-related macular degeneration, for which treatments remain limited, are among the most prevalent causes of legal blindness. Retinal prostheses have been developed to stimulate the inner retinal network; however, lack of sensitivity and resolution, and the need for wiring or external cameras, have limited their application. Here we show that conjugated polymer nanoparticles (P3HT NPs) mediate light-evoked stimulation of retinal neurons and persistently rescue visual functions when subretinally injected in a rat model of retinitis pigmentosa. P3HT NPs spread out over the entire subretinal space and promote light-dependent activation of spared inner retinal neurons, recovering subcortical, cortical and behavioural visual responses in the absence of trophic effects or retinal inflammation. By conferring sustained light sensitivity to degenerate retinas after a single injection, and with the potential for high spatial resolution, P3HT NPs provide a new avenue in retinal prosthetics with potential applications not only in retinitis pigmentosa, but also in age-related macular degeneration.