학술논문
Chromosome-scale assembly of the yellow mealworm genome.
Document Type
Academic Journal
Author
Eleftheriou E; Génomique Métabolique, Genoscope, Institut François Jacob, Commissariat à l'Energie Atomique (CEA), CNRS, Univ Evry, Université Paris-Saclay, Université Paris-Saclay, Evry, 91057, France.; Aury JM; Génomique Métabolique, Genoscope, Institut François Jacob, Commissariat à l'Energie Atomique (CEA), CNRS, Univ Evry, Université Paris-Saclay, Université Paris-Saclay, Evry, 91057, France.; Vacherie B; Genoscope, Institut de biologie François Jacob, CEA, Université Paris-Saclay, Evry, 91057, France.; Istace B; Génomique Métabolique, Genoscope, Institut François Jacob, Commissariat à l'Energie Atomique (CEA), CNRS, Univ Evry, Université Paris-Saclay, Université Paris-Saclay, Evry, 91057, France.; Belser C; Génomique Métabolique, Genoscope, Institut François Jacob, Commissariat à l'Energie Atomique (CEA), CNRS, Univ Evry, Université Paris-Saclay, Université Paris-Saclay, Evry, 91057, France.; Noel B; Génomique Métabolique, Genoscope, Institut François Jacob, Commissariat à l'Energie Atomique (CEA), CNRS, Univ Evry, Université Paris-Saclay, Université Paris-Saclay, Evry, 91057, France.; Moret Y; Équipe Écologie Évolutive, UMR CNRS 6282 BioGéoSciences, Université de Bourgogne Franche-Comté, Dijon, 21000, France.; Rigaud T; Équipe Écologie Évolutive, UMR CNRS 6282 BioGéoSciences, Université de Bourgogne Franche-Comté, Dijon, 21000, France.; Berro F; Ynsect, Evry, 91000, France.; Gasparian S; Ynsect, Evry, 91000, France.; Labadie-Bretheau K; Genoscope, Institut de biologie François Jacob, CEA, Université Paris-Saclay, Evry, 91057, France.; Lefebvre T; Ynsect, Evry, 91000, France.; Madoui MA; Génomique Métabolique, Genoscope, Institut François Jacob, Commissariat à l'Energie Atomique (CEA), CNRS, Univ Evry, Université Paris-Saclay, Université Paris-Saclay, Evry, 91057, France.; Équipe Écologie Évolutive, UMR CNRS 6282 BioGéoSciences, Université de Bourgogne Franche-Comté, Dijon, 21000, France.; Service d'Etude des Prions et des Infections Atypiques (SEPIA), Institut François Jacob, Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Université Paris Saclay, Fontenay-aux-Roses, France.
Source
Publisher: European Commission Country of Publication: Belgium NLM ID: 9918230081006676 Publication Model: eCollection Cited Medium: Internet ISSN: 2732-5121 (Electronic) Linking ISSN: 27325121 NLM ISO Abbreviation: Open Res Eur Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Background: The yellow mealworm beetle, Tenebrio molitor , is a promising alternative protein source for animal and human nutrition and its farming involves relatively low environmental costs. For these reasons, its industrial scale production started this century. However, to optimize and breed sustainable new T. molitor lines, the access to its genome remains essential. Methods: By combining Oxford Nanopore and Illumina Hi-C data, we constructed a high-quality chromosome-scale assembly of T. molitor . Then, we combined RNA-seq data and available coleoptera proteomes for gene prediction with GMOVE. Results: We produced a high-quality genome with a N50 = 21.9Mb with a completeness of 99.5% and predicted 21,435 genes with a median size of 1,780 bp. Gene orthology between T. molitor and Tribolium castaneum showed a highly conserved synteny between the two coleoptera and paralogs search revealed an expansion of histones in the T. molitor genome. Conclusions: The present genome will greatly help fundamental and applied research such as genetic breeding and will contribute to the sustainable production of the yellow mealworm.
Competing Interests: Competing interests: Fabrice Berro, Sona Gasparian and Thomas Lefebvre work for Ynsect, a private company breeding yellow mealworms. Ynsect is also the coordinator of the H2020 FARMYNG project that supports this study. No competing interests were disclosed for the other co-others.
(Copyright: © 2022 Eleftheriou E et al.)
Competing Interests: Competing interests: Fabrice Berro, Sona Gasparian and Thomas Lefebvre work for Ynsect, a private company breeding yellow mealworms. Ynsect is also the coordinator of the H2020 FARMYNG project that supports this study. No competing interests were disclosed for the other co-others.
(Copyright: © 2022 Eleftheriou E et al.)