학술논문
Analysis of the In Vivo Function of Follicular Regulatory T (T FR ) Cells in the Regulation of Antibody Response.
Document Type
Academic Journal
Author
Luo L; School of Pharmacy, Nantong University, Nantong, Jiangsu, China.; Leavenworth JW; Department of Neurosurgery, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA. jleavenworth@uabmc.edu.; Department of Microbiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA. jleavenworth@uabmc.edu.; The O'Neal Comprehensive Cancer Center, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA. jleavenworth@uabmc.edu.
Source
Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
Subject
Language
English
Abstract
Follicular regulatory T (T FR ) cells, a subset of Foxp3 + regulatory T cells (Tregs), play an essential role in the regulation of germinal center (GC) response and humoral self-tolerance. Although it is generally accepted that T FR cells suppress GC antibody responses mediated by follicular helper T (T FH ) cells and B cells, the helper activity of T FR cells on GC responses has also been recently reported. Because of this, it is critical to develop specific assays that are able to precisely assess T FR cell function, particularly its in vivo activity, independent of differentiation and other Tregs. Here we describe an adoptive transfer approach in conjugation with flow cytometry and ELISA to evaluate the T FR cell function on T FH , B cells, and antibody response in vivo.
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)