학술논문
ZNF692 regulates nucleolar morphology by interacting with NPM1 and modifying its self-assembly properties.
Document Type
Academic Journal
Author
Brown IN; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Levario A; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Jiang C; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Stachera W; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Rodriguez E; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Hao YH; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Woodruff JB; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.; Lafita-Navarro MC; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA. Electronic address: MariadelCarmen.LafitaNavarro@UTSouthwestern.edu.; Conacci-Sorrell M; Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas, USA. Electronic address: Maralice.conaccisorrell@utsouthwestern.edu.
Source
Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE
Subject
Language
English
Abstract
The nucleolus, a membrane-less organelle, is responsible for ribosomal RNA transcription, ribosomal RNA processing, and ribosome assembly. Nucleolar size and number are indicative of a cell's protein synthesis rate and proliferative capacity, and abnormalities in the nucleolus have been linked to neurodegenerative diseases and cancer. In this study, we demonstrated that the nucleolar protein ZNF692 directly interacts with nucleophosmin 1 (NPM1). Knocking down ZNF692 resulted in the nucleolar redistribution of NPM1 in ring-like structures and reduced protein synthesis. Purified NPM1 forms spherical condensates in vitro but mixing it with ZNF692 produces irregular condensates more closely resembling living cell nucleoli. Our findings indicate that ZNF692, by interacting with NPM1, plays a critical role in regulating nucleolar architecture and function in living cells.
Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)