학술논문
RAB-35 and ARF-6 GTPases Mediate Engulfment and Clearance Following Linker Cell-Type Death.
Document Type
Academic Journal
Author
Kutscher LM; Laboratory of Developmental Genetics, The Rockefeller University, New York, NY 10065, USA.; Keil W; Laboratory of Developmental Genetics, The Rockefeller University, New York, NY 10065, USA; Center for Studies in Physics and Biology, The Rockefeller University, New York, NY 10065, USA.; Shaham S; Laboratory of Developmental Genetics, The Rockefeller University, New York, NY 10065, USA. Electronic address: shaham@rockefeller.edu.
Source
Publisher: Cell Press Country of Publication: United States NLM ID: 101120028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1551 (Electronic) Linking ISSN: 15345807 NLM ISO Abbreviation: Dev Cell Subsets: MEDLINE
Subject
Language
English
Abstract
Clearance of dying cells is essential for development and homeostasis. Conserved genes mediate apoptotic cell removal, but whether these genes control non-apoptotic cell removal is a major open question. Linker cell-type death (LCD) is a prevalent non-apoptotic developmental cell death process with features conserved from C. elegans to vertebrates. Using microfluidics-based long-term in vivo imaging, we show that unlike apoptotic cells, the C. elegans linker cell, which dies by LCD, is competitively phagocytosed by two neighboring cells, resulting in cell splitting. Subsequent cell elimination does not require apoptotic engulfment genes. Rather, we find that RAB-35 GTPase is a key coordinator of competitive phagocytosis onset and cell degradation. RAB-35 binds CNT-1, an ARF-6 GTPase activating protein, and removes ARF-6, a degradation inhibitor, from phagosome membranes. This facilitates phosphatidylinositol-4,5-bisphosphate removal from phagosome membranes, promoting phagolysosome maturation. Our studies suggest that RAB-35 and ARF-6 drive a conserved program eliminating cells dying by LCD.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
(Copyright © 2018 Elsevier Inc. All rights reserved.)