학술논문

Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone.
Document Type
Academic Journal
Author
Park DJ; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA. Electronic address: dpark@broadinstitute.org.; Dudas G; Institute of Evolutionary Biology, Ashworth Laboratories, University of Edinburgh, Edinburgh EH9 3FL, UK.; Wohl S; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Goba A; Kenema Government Hospital, Kenema, Sierra Leone.; Whitmer SL; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Andersen KG; Scripps Translational Science Institute, The Scripps Research Institute, 3344 N Torrey Pines Court, La Jolla, CA 92037, USA.; Sealfon RS; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.; Ladner JT; US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.; Kugelman JR; US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.; Matranga CB; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Winnicki SM; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Qu J; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Gire SK; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Gladden-Young A; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Jalloh S; Kenema Government Hospital, Kenema, Sierra Leone.; Nosamiefan D; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Yozwiak NL; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Moses LM; Tulane University, 1430 Tulane Avenue, SL-38, New Orleans, LA 70112, USA.; Jiang PP; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Lin AE; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Schaffner SF; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Bird B; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Towner J; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Mamoh M; Kenema Government Hospital, Kenema, Sierra Leone.; Gbakie M; Kenema Government Hospital, Kenema, Sierra Leone.; Kanneh L; Kenema Government Hospital, Kenema, Sierra Leone.; Kargbo D; Kenema Government Hospital, Kenema, Sierra Leone.; Massally JL; Kenema Government Hospital, Kenema, Sierra Leone.; Kamara FK; Kenema Government Hospital, Kenema, Sierra Leone.; Konuwa E; Kenema Government Hospital, Kenema, Sierra Leone.; Sellu J; Kenema Government Hospital, Kenema, Sierra Leone.; Jalloh AA; Kenema Government Hospital, Kenema, Sierra Leone.; Mustapha I; Kenema Government Hospital, Kenema, Sierra Leone.; Foday M; Kenema Government Hospital, Kenema, Sierra Leone.; Yillah M; Kenema Government Hospital, Kenema, Sierra Leone.; Erickson BR; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Sealy T; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Blau D; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Paddock C; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Brault A; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Amman B; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Basile J; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Bearden S; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Belser J; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Bergeron E; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Campbell S; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Chakrabarti A; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Dodd K; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Flint M; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Gibbons A; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Goodman C; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Klena J; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; McMullan L; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Morgan L; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Russell B; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Salzer J; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Sanchez A; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Wang D; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Jungreis I; Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.; Tomkins-Tinch C; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Kislyuk A; DNAnexus, 1975 West El Camino Real, Suite 101, Mountain View, CA 94040, USA.; Lin MF; DNAnexus, 1975 West El Camino Real, Suite 101, Mountain View, CA 94040, USA.; Chapman S; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; MacInnis B; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Matthews A; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA.; Bochicchio J; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Hensley LE; Integrated Research Facility at Fort Detrick, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, B-8200 Research Plaza, Fort Detrick, Frederick, MD 21702, USA.; Kuhn JH; Integrated Research Facility at Fort Detrick, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, B-8200 Research Plaza, Fort Detrick, Frederick, MD 21702, USA.; Nusbaum C; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Schieffelin JS; Tulane University, 1430 Tulane Avenue, SL-38, New Orleans, LA 70112, USA.; Birren BW; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Forget M; Médecins Sans Frontières, Rue de l'Arbre Bénit 46, 1050 Bruxelles, Belgium.; Nichol ST; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Palacios GF; US Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702, USA.; Ndiaye D; Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal.; Happi C; Redeemers University Nigeria, KM 46 Lagos-Ibadan Expressway, Redemption City, Ogun State, Nigeria.; Gevao SM; University of Sierra Leone, A.J. Momoh St, Tower Hill, Freetown, Sierra Leone.; Vandi MA; Sierra Leone Ministry of Health and Sanitation, Youyi Building, Freetown, Sierra Leone.; Kargbo B; Sierra Leone Ministry of Health and Sanitation, Youyi Building, Freetown, Sierra Leone.; Holmes EC; University of Sydney, Johns Hopkins Drive, Camperdown NSW 2050, Australia.; Bedford T; Fred Hutchinson Cancer Research Center, 110 Fairview Avenue North, Seattle, WA 98109, USA.; Gnirke A; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA.; Ströher U; National Center for Emerging and Zoonotic Infectious Diseases and National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Mailstop-G14, Atlanta, GA 30333, USA.; Rambaut A; Institute of Evolutionary Biology, Ashworth Laboratories, University of Edinburgh, Edinburgh EH9 3FL, UK; Centre for Immunology, Infection and Evolution, University of Edinburgh, Ashworth Laboratories, Edinburgh EH9 3FL, UK; Fogarty International Center, National Institutes of Health, 31 Center Drive, MSC 2220 Bethesda, MD 20892, USA. Electronic address: a.rambaut@ed.ac.uk.; Garry RF; Tulane University, 1430 Tulane Avenue, SL-38, New Orleans, LA 70112, USA.; Sabeti PC; Broad Institute of Harvard and MIT, 75 Ames Street, Cambridge, MA 02142, USA; Harvard University, 52 Oxford Street, Cambridge, MA 02138, USA. Electronic address: pardis@broadinstitute.org.
Source
Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE
Subject
Language
English
Abstract
The 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
(Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)