학술논문
Development of DNA aptamers for visualization of glial brain tumors and detection of circulating tumor cells.
Document Type
Academic Journal
Author
Kichkailo AS; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Narodov AA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Krasnoyarsk Inter-District Ambulance Hospital named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia.; Komarova MA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Zamay TN; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Zamay GS; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Kolovskaya OS; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Erakhtin EE; Krasnoyarsk Inter-District Ambulance Hospital named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia.; Glazyrin YE; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Veprintsev DV; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Moryachkov RV; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Zabluda VV; Laboratory of Physics of Magnetic Phenomena, Kirensky Institute of Physics, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia.; Shchugoreva I; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Siberian Federal University, 79 Svobodny pr., Krasnoyarsk 660041, Russia.; Artyushenko P; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Siberian Federal University, 79 Svobodny pr., Krasnoyarsk 660041, Russia.; Mironov VA; Department of Chemistry, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 702-701, South Korea.; Morozov DI; Nanoscience Center and Department of Chemistry, University of Jyväskylä, P.O. Box 35, Jyväskylä 40014, Finland.; Khorzhevskii VA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Krasnoyarsk Regional Pathology-Anatomic Bureau, 3d Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Gorbushin AV; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Krasnoyarsk Inter-District Ambulance Hospital named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia.; Koshmanova AA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Nikolaeva ED; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Grinev IP; Krasnoyarsk Inter-District Ambulance Hospital named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia.; Voronkovskii II; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Krasnoyarsk Inter-District Ambulance Hospital named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia.; Grek DS; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Belugin KV; Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk, Russia.; Volzhentsev AA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Badmaev ON; Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk, Russia.; Luzan NA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Lukyanenko KA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Siberian Federal University, 79 Svobodny pr., Krasnoyarsk 660041, Russia.; Peters G; National Research Center Kurchatov Institute, 1 Akademika Kurchatova, Moscow 123182, Russia.; Lapin IN; Laboratory of Advanced Materials and Technology, Siberian Physical-Technical Institute of Tomsk State University, 36 Lenina, Tomsk 634050, Russia.; Kirichenko AK; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Konarev PV; National Research Center Kurchatov Institute, 1 Akademika Kurchatova, Moscow 123182, Russia.; A.V. Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' RAS, 59 Leninsky pr., Moscow 119333, Russia.; Morozov EV; Institute of Chemistry and Chemical Technology SB RAS - The Branch of Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', Krasnoyarsk 660036, Russia.; Mironov GG; Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON K1N6N5, Canada.; Gargaun A; Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON K1N6N5, Canada.; Muharemagic D; Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON K1N6N5, Canada.; Zamay SS; Department of Molecular Electronics, Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Kochkina EV; Siberian Federal University, 79 Svobodny pr., Krasnoyarsk 660041, Russia.; Dymova MA; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 Lavrentyev Avenue, Novosibirsk 630090, Russia.; Smolyarova TE; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Sokolov AE; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences,' 50 Akademgorodok, Krasnoyarsk 660036, Russia.; Laboratory of Physics of Magnetic Phenomena, Kirensky Institute of Physics, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia.; Modestov AA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Tokarev NA; Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk, Russia.; Shepelevich NV; Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk, Russia.; Ozerskaya AV; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk, Russia.; Chanchikova NG; Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk, Russia.; Krat AV; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Krasnoyarsk Regional Clinical Cancer Center, 16 1-ya Smolenskaya, Krasnoyarsk 660133, Russia.; Zukov RA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Krasnoyarsk Regional Clinical Cancer Center, 16 1-ya Smolenskaya, Krasnoyarsk 660133, Russia.; Bakhtina VI; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Shnyakin PG; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Shesternya PA; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Svetlichnyi VA; Laboratory of Advanced Materials and Technology, Siberian Physical-Technical Institute of Tomsk State University, 36 Lenina, Tomsk 634050, Russia.; Petrova MM; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Artyukhov IP; Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizana Zheleznyaka, Krasnoyarsk 660022, Russia.; Tomilin FN; Laboratory of Physics of Magnetic Phenomena, Kirensky Institute of Physics, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia.; Siberian Federal University, 79 Svobodny pr., Krasnoyarsk 660041, Russia.; Berezovski MV; Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie-Curie, Ottawa, ON K1N6N5, Canada.
Source
Publisher: Cell Press Country of Publication: United States NLM ID: 101581621 Publication Model: eCollection Cited Medium: Print ISSN: 2162-2531 (Print) Linking ISSN: 21622531 NLM ISO Abbreviation: Mol Ther Nucleic Acids Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2162-2531
Abstract
Here, we present DNA aptamers capable of specific binding to glial tumor cells in vitro , ex vivo , and in vivo for visualization diagnostics of central nervous system tumors. We selected the aptamers binding specifically to the postoperative human glial primary tumors and not to the healthy brain cells and meningioma, using a modified process of systematic evolution of ligands by exponential enrichment to cells; sequenced and analyzed ssDNA pools using bioinformatic tools and identified the best aptamers by their binding abilities; determined three-dimensional structures of lead aptamers (Gli-55 and Gli-233) with small-angle X-ray scattering and molecular modeling; isolated and identified molecular target proteins of the aptamers by mass spectrometry; the potential binding sites of Gli-233 to the target protein and the role of post-translational modifications were verified by molecular dynamics simulations. The anti-glioma aptamers Gli-233 and Gli-55 were used to detect circulating tumor cells in liquid biopsies. These aptamers were used for in situ , ex vivo tissue staining, histopathological analyses, and fluorescence-guided tumor and PET/CT tumor visualization in mice with xenotransplanted human astrocytoma. The aptamers did not show in vivo toxicity in the preclinical animal study. This study demonstrates the potential applications of aptamers for precise diagnostics and fluorescence-guided surgery of brain tumors.
Competing Interests: The authors declare no competing interests.
(© 2023 The Authors.)
Competing Interests: The authors declare no competing interests.
(© 2023 The Authors.)