학술논문
Performance of Dried Blood Spots Compared with Serum Samples for Measuring Dengue Seroprevalence in a Cohort of Children in Cebu, Philippines.
Document Type
Academic Journal
Author
Daag JV; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.; Ylade M; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.; Jadi R; 2Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.; Adams C; 2Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.; Cuachin AM; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.; Alpay R; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.; Aportadera ETC; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.; Yoon IK; 3Coalition for Epidemic Preparedness Innovations, Washington, District of Columbia.; de Silva AM; 2Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina.; Lopez AL; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.; Deen J; 1Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines-Manila, Manila, Philippines.
Source
Publisher: American Society of Tropical Medicine and Hygiene Country of Publication: United States NLM ID: 0370507 Publication Model: Print Cited Medium: Internet ISSN: 1476-1645 (Electronic) Linking ISSN: 00029637 NLM ISO Abbreviation: Am J Trop Med Hyg Subsets: MEDLINE
Subject
Language
English
Abstract
Dengue seroprevalence data are useful for understanding epidemiologic trends and transmission dynamics, and for making decisions about implementation of dengue control programs. A logistical challenge to seroprevalence surveys is the collection and transport of serum samples. For conducting large and repeated dengue serosurveys, dried blood spots (DBS) would allow easier sample collection, shipment, transport, and storage than standard serum collection methods. Further evidence is needed to understand how well DBS performs compared with standard serum collection methods in laboratory assays. We evaluated the detection of anti-dengue antibodies by IgG indirect ELISA when using DBS compared with sera. Specimens were collected from healthy children in Cebu, Philippines, who would be 9-14 years of age at the time of a mass dengue vaccination program. Using an ELISA index value cutoff of 0.9, 1,285/1,488 (86.4%) of the DBS were seropositive and 203 (13.6%) were seronegative, compared with 1,292/1,488 (86.8%) seropositive and 196 (13.2%) seronegative serum samples. Compared with sera, the DBS method had a 98.3% sensitivity, 92.4% specificity, 98.9% positive predictive value, and 89.2% negative predictive value. Considering the advantages in terms of sample collection, shipment, and storage, DBS sampling may be appropriate for dengue population serosurveys.