학술논문
DNA Quantity and Quality Comparisons between Cryopreserved and FFPE Tumors from Matched Pan-Cancer Samples.
Document Type
Academic Journal
Author
Okojie J; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA.; O'Neal N; Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA.; Burr M; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Worley P; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Packer I; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Anderson D; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Davis J; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Kearns B; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Fatema K; Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA.; Dixon K; Specicare, 690 Medical Park Ln, Gainesville, GA 30501, USA.; Barrott JJ; Department of Cell Biology & Physiology, Brigham Young University, Provo, UT 84602, USA.; Department of Biomedical and Pharmaceutical Sciences, Idaho State University, Pocatello, ID 83209, USA.; Specicare, 690 Medical Park Ln, Gainesville, GA 30501, USA.; Simmons Center for Cancer Research, Brigham Young University, Provo, UT 84602, USA.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 9502503 Publication Model: Electronic Cited Medium: Internet ISSN: 1718-7729 (Electronic) Linking ISSN: 11980052 NLM ISO Abbreviation: Curr Oncol Subsets: MEDLINE
Subject
Language
English
Abstract
Personalized cancer care requires molecular characterization of neoplasms. While the research community accepts frozen tissues as the gold standard analyte for molecular assays, the source of tissue for testing in clinical cancer care comes almost universally from formalin-fixed, paraffin-embedded tissue (FFPE). As newer technologies emerge for DNA characterization that requires higher molecular weight DNA, it was necessary to compare the quality of DNA in terms of DNA length between FFPE and cryopreserved samples. We hypothesized that cryopreserved samples would yield higher quantity and superior quality DNA compared to FFPE samples. We analyzed DNA metrics by performing a head-to-head comparison between FFPE and cryopreserved samples from 38 human tumors representing various cancer types. DNA quantity and purity were measured by UV spectrophotometry, and DNA from cryopreserved tissue demonstrated a 4.2-fold increase in DNA yield per mg of tissue ( p -value < 0.001). DNA quality was measured on a fragment microelectrophoresis analyzer, and again, DNA from cryopreserved tissue demonstrated a 223% increase in the DNA quality number and a 9-fold increase in DNA fragments > 40,000 bp ( p -value < 0.0001). DNA from the cryopreserved tissues was superior to the DNA from FFPE samples in terms of DNA yield and quality.