학술논문
Dengue virus IgG and serotype-specific neutralizing antibody titers measured with standard and mature viruses are associated with protection.
Document Type
Author
Katzelnick L; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Odio C; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Daag J; Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines Manila, Manila, Philippines.; Crisostomo MV; Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines Manila, Manila, Philippines.; Voirin C; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Escoto AC; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Adams C; University of North Carolina at Chapel Hill.; Hein LD; Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill.; Aogo R; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Mpingabo P; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Rodriguez GR; Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health.; Firdous S; National Institute of Allergy and Infectious Diseases.; Fernandez MA; Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill.; White L; Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill.; Agrupis KA; Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines Manila.; Deen J; Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines Manila.; de Silva A; University of North Carolina at Chapel Hill.; Ylade M; Institute of Child Health and Human Development, National Institutes of Health, University of the Philippines Manila.
Source
Country of Publication: United States NLM ID: 101768035 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: Res Sq Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Recent work demonstrates the limitations of the standard dengue virus (DENV) neutralization assay to predict protection against dengue. We perform studies to compare how a commercial IgG ELISA, envelope domain III (EDIII) or non-structural protein 1 (NS1) binding antibodies, and titers from plaque reduction neutralization tests (PRNTs) using reference standard and clinical mature viruses are associated with dengue disease. Healthy children (n = 1,206) in Cebu, Philippines were followed for 5 years. High ELISA values (≥3) were associated with reduced dengue probability relative to naïve children (3% vs. 10%, p = 0.008), but antibody binding EDIII or NS1 from each serotype had no association. High standard and mature geometric mean PRNT titers were associated with reduced dengue disease overall (p < 0.01), and high DENV2 and DENV3 titers in both assays provided protection against the matched serotype (p < 0.02). However, while 52% of dengue cases had standard virus PRNT titers > 100, only 2% of cases had mature virus PRNT titers > 100 (p < 0.001), indicating a lower, more consistent threshold for protection. Each assay may be useful for different purposes as correlates of protection in population and vaccine trials.
Competing Interests: Declaration of Interests: The authors declare no conflicts of interest.
Competing Interests: Declaration of Interests: The authors declare no conflicts of interest.