학술논문
Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith. from the semi-arid region of Northeastern Brazil.
Document Type
Academic Journal
Author
de Veras BO; Post-graduation in Tropical Medicine, Laboratory of Microbiology, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil; Post-graduation in Biotechnology - RENORBIO, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil; Department of Biochemistry, Laboratory of Natural Products, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil. Electronic address: bruno.overas@ufpe.br.; Moura GMM; Department of Biochemistry, Laboratory Chemistry and Function of Bioactive Proteins, Federal University of Rio Grande do Norte, 59078-970, Natal, Rio Grande do Norte, Brazil.; Barros AV; Department of Biochemistry, Laboratory of Natural Products, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.; Vanusa da Silva M; Department of Biochemistry, Laboratory of Natural Products, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.; Assis PAC; Department of Physiology and Pathology, Laboratory of Immunology and Cell Biology, Federal University of Paraiba, 58051-900, João Pessoa, Paraiba, Brazil.; Aguiar JCROF; Department of Chemistry, Center for Exact and Nature Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.; Navarro DMDAF; Department of Chemistry, Center for Exact and Nature Sciences, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.; Ximenes RM; Post-graduation in Biotechnology - RENORBIO, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.; Wanderley AG; Department of Pharmaceutical Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, 09913-030, Diadema, São Paulo, Brazil.; Oliveira MBM; Department of Biochemistry, Laboratory of Natural Products, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.; Lopes ACS; Post-graduation in Tropical Medicine, Laboratory of Microbiology, Federal University of Pernambuco, 50670-420, Recife, Pernambuco, Brazil.
Source
Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE
Subject
Language
English
Abstract
Ethnopharmacological Relevance: Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro". In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain. However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
Aim of the Study: This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
Material and Methods: The acute toxicity of the essential oil was investigated in mice. The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception. The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
Results: No acute toxicity was observed at doses up to 2000 mg/kg; p.o. The antinociceptive effect was statistically equal to morphine. In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP). In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed. The antipyretic effect was statistically superior to dipyrone. The reduction in paw edema was statistically superior to the standard in both models.
Conclusion: The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Aim of the Study: This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
Material and Methods: The acute toxicity of the essential oil was investigated in mice. The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception. The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
Results: No acute toxicity was observed at doses up to 2000 mg/kg; p.o. The antinociceptive effect was statistically equal to morphine. In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP). In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed. The antipyretic effect was statistically superior to dipyrone. The reduction in paw edema was statistically superior to the standard in both models.
Conclusion: The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)