학술논문
Synthesis and Characterization of a Biocompatible Nanoplatform Based on Silica-Embedded SPIONs Functionalized with Polydopamine.
Document Type
Academic Journal
Author
Romano M; Department of Molecular and Translational Medicine, University of Brescia, Brescia25123, Italy.; Center for Colloid and Surface Science (CSGI), Florence50019, Italy.; Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg85764, Germany.; González Gómez MA; NANOMAG Laboratory, Applied Physics Department, iMATUS Materials Institute, Universidade de Santiago de Compostela, Santiago de Compostela15782, Spain.; Santonicola P; Institute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), Naples80131, Italy.; Aloi N; Institute for Biomedical Research and Innovation (IRIB), National Research Council of Italy (CNR), Palermo90146, Italy.; Offer S; Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg85764, Germany.; Pantzke J; Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg85764, Germany.; Raccosta S; Institute of Biophysics (IBF), National Research Council of Italy (CNR), Palermo90146, Italy.; Longo V; Institute for Biomedical Research and Innovation (IRIB), National Research Council of Italy (CNR), Palermo90146, Italy.; Surpi A; Institute of Nanostructured Materials (ISMN), National Research Council of Italy (CNR), Bologna40129, Italy.; Alacqua S; Department of Molecular and Translational Medicine, University of Brescia, Brescia25123, Italy.; Center for Colloid and Surface Science (CSGI), Florence50019, Italy.; Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg85764, Germany.; Zampi G; Institute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), Naples80131, Italy.; Dediu VA; Institute of Nanostructured Materials (ISMN), National Research Council of Italy (CNR), Bologna40129, Italy.; Michalke B; Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg85764, Germany.; Zimmerman R; Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg85764, Germany.; Manno M; Institute of Biophysics (IBF), National Research Council of Italy (CNR), Palermo90146, Italy.; Piñeiro Y; NANOMAG Laboratory, Applied Physics Department, iMATUS Materials Institute, Universidade de Santiago de Compostela, Santiago de Compostela15782, Spain.; Colombo P; Institute for Biomedical Research and Innovation (IRIB), National Research Council of Italy (CNR), Palermo90146, Italy.; Di Schiavi E; Institute of Biosciences and BioResources (IBBR), National Research Council of Italy (CNR), Naples80131, Italy.; Rivas J; NANOMAG Laboratory, Applied Physics Department, iMATUS Materials Institute, Universidade de Santiago de Compostela, Santiago de Compostela15782, Spain.; Bergese P; Department of Molecular and Translational Medicine, University of Brescia, Brescia25123, Italy.; Center for Colloid and Surface Science (CSGI), Florence50019, Italy.; Di Bucchianico S; Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg85764, Germany.
Source
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101654670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-9878 (Electronic) Linking ISSN: 23739878 NLM ISO Abbreviation: ACS Biomater Sci Eng Subsets: MEDLINE
Subject
Language
English
Abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) have gained increasing interest in nanomedicine, but most of those that have entered the clinical trials have been withdrawn due to toxicity concerns. Therefore, there is an urgent need to design low-risk and biocompatible SPION formulations. In this work, we present an original safe-by-design nanoplatform made of silica nanoparticles loaded with SPIONs and decorated with polydopamine (SPIONs@SiO2-PDA) and the study of its biocompatibility performance by an ad hoc thorough in vitro to in vivo nanotoxicological methodology. The results indicate that the SPIONs@SiO 2 -PDA have excellent colloidal stability in serum-supplemented culture media, even after long-term (24 h) exposure, showing no cytotoxic or genotoxic effects in vitro and ex vivo. Physiological responses, evaluated in vivo using Caenorhabditis elegans as the animal model, showed no impact on fertility and embryonic viability, induction of an oxidative stress response, and a mild impact on animal locomotion. These tests indicate that the synergistic combination of the silica matrix and PDA coating we developed effectively protects the SPIONs, providing enhanced colloidal stability and excellent biocompatibility.