학술논문
Impact of Multiplex PCR in Reducing the Risk of Residual Transfusion-Transmitted Human Immunodeficiency and Hepatitis B and C Viruses in Burkina Faso.
Document Type
Academic Journal
Author
Yooda AP; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.; National Blood Transfusion Center (CNTS), 01 BP 5372 Ouagadougou 01, Burkina Faso.; Soubeiga ST; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.; Nebie KY; National Blood Transfusion Center (CNTS), 01 BP 5372 Ouagadougou 01, Burkina Faso.; Diarra B; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Sawadogo S; National Blood Transfusion Center (CNTS), 01 BP 5372 Ouagadougou 01, Burkina Faso.; Ouattara AK; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.; Obiri-Yeboah D; Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Ghana.; Yonli AT; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.; Tao I; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.; Sorgho PA; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.; Dahourou H; National Blood Transfusion Center (CNTS), 01 BP 5372 Ouagadougou 01, Burkina Faso.; Simpore J; Laboratory of Molecular Biology and Molecular Genetics (LABIOGENE) UFR/SVT, University Ouaga I Prof. Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.; Biomolecular Research Center Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.
Source
Publisher: Università Cattolica del Sacro Cuore Country of Publication: Italy NLM ID: 101530512 Publication Model: eCollection Cited Medium: Print ISSN: 2035-3006 (Print) Linking ISSN: 20353006 NLM ISO Abbreviation: Mediterr J Hematol Infect Dis Subsets: PubMed not MEDLINE
Subject
Language
English
ISSN
2035-3006
Abstract
Background and Objective: The improved performance of serological tests has significantly reduced the risk of human immunodeficiency and hepatitis B and C viruses transmission by blood transfusion, but there is a persistence of residual risk. The objective of this study was to evaluate the impact of multiplex PCR in reducing the risk of residual transmission of these viruses in seronegative blood donors in Burkina Faso.
Methods: This cross-sectional study was conducted from March to September 2017. The serological tests were performed on sera using ARCHITECTSR i 1000 (Abbot diagnosis, USA). Detection of viral nucleic acids was performed by multiplex PCR on mini-pools of seronegative plasma for HBV, HCV and HIV using SaCycler-96 Real Time PCR v.7.3 (Sacace Biotechnologies). Multiplex PCR-positive samples from these mini-pools were then individually tested by the same method.
Results: A total of 989 donors aged 17 to 65 were included in the present study. "Repeat donors" accounted for 44.79% (443/989). Seroprevalences for HIV, HBV, and HCV were 2.53% (25/989), 7.28% (72/989) and 2.73% (27/989), respectively. Of the 14 co-infections detected, HBV/HCV was the most common with 0.71% (7/989) of cases. Of 808 donations tested by multiplex PCR, 4.70% (38/808) were positive for HBV while no donation was positive for HIV or HCV.
Conclusion: Our study showed a high residual risk of HBV transmission through blood transfusion. Due to the high prevalence of blood-borne infections in Burkina Faso, we recommend the addition of multiplex PCR to serologic tests for optimal blood donation screening.
Competing Interests: Competing interests: The authors have declared that no competing interests exist.
Methods: This cross-sectional study was conducted from March to September 2017. The serological tests were performed on sera using ARCHITECT
Results: A total of 989 donors aged 17 to 65 were included in the present study. "Repeat donors" accounted for 44.79% (443/989). Seroprevalences for HIV, HBV, and HCV were 2.53% (25/989), 7.28% (72/989) and 2.73% (27/989), respectively. Of the 14 co-infections detected, HBV/HCV was the most common with 0.71% (7/989) of cases. Of 808 donations tested by multiplex PCR, 4.70% (38/808) were positive for HBV while no donation was positive for HIV or HCV.
Conclusion: Our study showed a high residual risk of HBV transmission through blood transfusion. Due to the high prevalence of blood-borne infections in Burkina Faso, we recommend the addition of multiplex PCR to serologic tests for optimal blood donation screening.
Competing Interests: Competing interests: The authors have declared that no competing interests exist.