학술논문
Oroxylin A inhibits inflammatory cytokines in periodontitis via HO‑1.
Document Type
Academic Journal
Author
Wang T; Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.; Wang ZB; Department of Medical Imaging, Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital) Qingdao, Shandong 266003, P.R. China.; Jiang CM; Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.; Zhao Y; Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.; Tang L; Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.; Xiao XM; Department of Endodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.; Fu J; Department of Prosthodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Source
Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
Subject
Language
English
Abstract
Periodontal disease is a common infectious disease that can lead to the loss of teeth. Hower how to effectively suppress the inflammation with medication is unclear. The aim of the present study was to investigate the anti‑inflammatory effect of Oroxylin A in periodontitis and its potential role through heme oxygenase‑1 (HO‑1). Primary rat gingival fibroblasts (RGFs) were cultured using the tissue block method and identified by immunofluorescence. Following lipopolysaccharide (LPS) stimulation of RGFs, Oroxylin A was administered at 50, 100, 200 or 400 µg/ml. Reverse transcription‑quantitative PCR was used to assess mRNA expression of cyclooxygenase (COX)‑2, TNF‑α, RANKL and osteoprotegerin (OPG). Western blotting was used to detect protein expression levels of COX ‑2, TNF‑α, RANKL and OPG. Following HO‑1 knockdown, the same treatment was performed. The expression of COX‑2 in rat gingival tissue was observed by immunohistochemistry. One‑way analysis of variance and Student's t test were used for statistical analysis. Oroxylin A downregulated mRNA expression of COX‑2, TNF‑α, RANKL and OPG in LPS‑induced RGFs. With increase of Oroxylin A dose, the expression of HO‑1 was gradually upregulated. When HO‑1 was knocked down, Oroxylin A did not downregulate the expression of COX‑2, TNF‑α, RANKL and OPG in LPS‑induced RGFs. Immunohistochemical results showed that expression of COX‑2 was downregulated by Oroxylin A, and the expression of TNF‑α, RANKL and OPG were also downregulated. Oroxylin A decreased expression of inflammatory cytokines in LPS‑induced RGFs and had a good inhibitory effect on periodontitis in rats.