학술논문
Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method.
Document Type
Academic Journal
Author
Kikuchi Y; Faculty of Life and Environmental Science, University of Yamanashi, Kofu, 400-8510, Japan.; Ito D; Faculty of Life and Environmental Science, University of Yamanashi, Kofu, 400-8510, Japan.; Wakayama S; Advanced Biotechnology Center, University of Yamanashi, Kofu, 400-8510, Japan.; Ooga M; Faculty of Life and Environmental Science, University of Yamanashi, Kofu, 400-8510, Japan.; Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, Fuchinobe, Chuo-ku, Sagamihara, 252-5201, Japan.; Wakayama T; Advanced Biotechnology Center, University of Yamanashi, Kofu, 400-8510, Japan. twakayama@yamanashi.ac.jp.
Source
Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Subject
Language
English
Abstract
Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube that does not require a CO 2 incubator. In the present study, we developed a new time-lapse observation system using our previous culture method and a glass capillary. Zygotes were placed in a glass capillary and sunk in oil for observation under a stereomicroscope. Warming the capillary using a thermoplate enabled most of the zygotes to develop into blastocysts and produce healthy offspring. This time-lapse observation system captured images every 30 min for up to 5 days, which confirmed that the developmental speed and quality of the embryos were not affected, even with fluorescence. Overall, this new system is a simple time-lapse observation method for preimplantation embryos that does not require dedicated machines and advanced techniques.
(© 2023. The Author(s).)
(© 2023. The Author(s).)