학술논문
Injection parameters affect cell viability and implant volumes in automated cell delivery for the brain.
Document Type
Academic Journal
Author
Kondziolka D; Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA. kondziolkads@upmc.edu; Gobbel GT; Fellows-Mayle W; Chang YF; Uram M
Source
Publisher: SAGE Publishing Country of Publication: United States NLM ID: 9208854 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1555-3892 (Electronic) Linking ISSN: 09636897 NLM ISO Abbreviation: Cell Transplant Subsets: MEDLINE
Subject
Language
English
Abstract
The technique of central nervous system cell implantation can affect the outcome of preclinical or clinical studies. Our goal was to evaluate the impact of various injection parameters that may be of consequence during the delivery of solute-suspended cells. These parameters included (1) the type and concentration of cells used for implantation, (2) the rate at which cells are injected (flow rate), (3) the acceleration of the delivery device, (4) the period of time between cell loading and injection into the CNS (delay), and (5) the length and gauge of the needle used to deliver the cells. Neural progenitor cells (NPCs) and bone marrow stromal cells (BMSCs) were injected an automated device. These parameters were assessed in relation to their effect on the volume of cells injected and cell viability. Longer and thinner cannulae and higher cell concentrations were detrimental for cell delivery. Devices and techniques that optimize these parameters should be of benefit.