학술논문
Edible exosome-like nanoparticles from portulaca oleracea L mitigate DSS-induced colitis via facilitating double-positive CD4 + CD8 + T cells expansion.
Document Type
Academic Journal
Author
Zhu MZ; Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China.; Xu HM; Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China.; Liang YJ; School of Rehabilitation Medicine, Jining Medical University, Jining, 272029, Shandong, China.; Xu J; Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China.; Yue NN; Department of Gastroenterology, Shenzhen People's Hospital, the Second Clinical Medical College, Jinan University, Shenzhen, 518020, Guangdong, China.; Zhang Y; Department of Medical Administration, Huizhou Institute of Occupational Diseases Control and Prevention, Huizhou, 516000, Guangdong, China.; Tian CM; Department of Emergency, Shenzhen People's Hospital, the Second Clinical Medical College, the First Affiliated Hospital, Jinan University, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China.; Yao J; Department of Gastroenterology, Shenzhen People's Hospital, the Second Clinical Medical College, the First Affiliated Hospital, Jinan University, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China. yao.jun@szhospital.com.; Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People's Hospital, Shenzhen, 518020, Guangdong, China. yao.jun@szhospital.com.; Wang LS; Department of Gastroenterology, Shenzhen People's Hospital, the Second Clinical Medical College, the First Affiliated Hospital, Jinan University, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China. wanglsszrmyy@163.com.; Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People's Hospital, Shenzhen, 518020, Guangdong, China. wanglsszrmyy@163.com.; Nie YQ; Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, 510006, Guangdong, China. eynieyuqiang@scut.edu.cn.; Li DF; Department of Gastroenterology, Shenzhen People's Hospital, the Second Clinical Medical College, the First Affiliated Hospital, Jinan University, Southern University of Science and Technology, Shenzhen, 518020, Guangdong, China. ldf830712@163.com.; Shenzhen Clinical Research Centre for Geriatrics, Shenzhen People's Hospital, Shenzhen, 518020, Guangdong, China. ldf830712@163.com.
Source
Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE
Subject
Language
English
Abstract
Plant-derived exosome-like nanoparticles (PDENs) have been paid great attention in the treatment of ulcerative colitis (UC). As a proof of concept, we isolated and identified Portulaca oleracea L-derived exosome-like nanoparticles (PELNs) from edible Portulaca oleracea L, which exhibited desirable nano-size (~ 160 nm) and a negative zeta potential value (-31.4 mV). Oral administration of PELNs effectively suppressed the expressions of pro-inflammatory cytokines (TNF-α, IL-6, IL-12, and IL-1β) and myeloperoxidase (MPO), increased levels of the anti-inflammatory cytokine (IL-10), and alleviated acute colitis in dextran sulfate sodium (DSS)-induced C57 mice and IL-10 -/- mice. Notably, PELNs exhibited excellent stability and safety within the gastrointestinal tract and displayed specific targeting to inflamed sites in the colons of mice. Mechanistically, oral administration of PELNs played a crucial role in maintaining the diversity and balance of gut microbiota. Furthermore, PELNs treatment enhanced Lactobacillus reuteri growth and elevated indole derivative levels, which might activate the aryl-hydrocarbon receptor (AhR) in conventional CD4 + T cells. This activation downregulated Zbtb7b expression, leading to the reprogramming of conventional CD4 + T cells into double-positive CD4 + CD8 + T cells (DP CD4 + CD8 + T cells). In conclusion, our findings highlighted the potential of orally administered PELNs as a novel, natural, and colon-targeted agent, offering a promising therapeutic approach for managing UC. Schematic illustration of therapeutic effects of oral Portulaca oleracea L -derived natural exosome-like nanoparticles (PELNs) on UC. PELNs treatment enhanced Lactobacillus reuteri growth and elevated indole derivative levels, which activate the aryl-hydrocarbon receptor (AhR) in conventional CD4 + T cells leading to downregulate the expression of Zbtb7b, reprogram of conventional CD4 + T cells into double-positive CD4 + CD8 + T cells (DP CD4 + CD8 + T cells), and decrease the levels of pro-inflammatory cytokines.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
(© 2023. BioMed Central Ltd., part of Springer Nature.)