학술논문
Osmoregulation determines sperm cell geometry and integrity for double fertilization in flowering plants.
Document Type
Academic Journal
Author
Chen SY; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.; Wang L; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.; Jia PF; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.; Yang WC; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.; Sze H; Department of Cell Biology & Molecular Genetics, University of Maryland, College Park, MD 20742, USA.; Li HJ; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: hjli@genetics.ac.cn.
Source
Publisher: Cell Press Country of Publication: England NLM ID: 101465514 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1752-9867 (Electronic) Linking ISSN: 16742052 NLM ISO Abbreviation: Mol Plant Subsets: MEDLINE
Subject
Language
English
Abstract
Distinct from the motile flagellated sperm of animals and early land plants, the non-motile sperm cells of flowering plants are carried in the pollen grain to the female pistil. After pollination, a pair of sperm cells are delivered into the embryo sac by pollen tube growth and rupture. Unlike other walled plant cells with an equilibrium between internal turgor pressure and mechanical constraints of the cell walls, sperm cells wrapped inside the cytoplasm of a pollen vegetative cell have only thin and discontinuous cell walls. The sperm cells are uniquely ellipsoid in shape, although it is unclear how they maintain this shape within the pollen tubes and after release. In this study, we found that genetic disruption of three endomembrane-associated cation/H + exchangers specifically causes sperm cells to become spheroidal in hydrated pollens of Arabidopsis. Moreover, the released mutant sperm cells are vulnerable and rupture before double fertilization, leading to failed seed set, which can be partially rescued by depletion of the sperm-expressed vacuolar water channel. These results suggest a critical role of cell-autonomous osmoregulation in adjusting the sperm cell shape for successful double fertilization in flowering plants.
(Copyright © 2022 The Author. Published by Elsevier Inc. All rights reserved.)
(Copyright © 2022 The Author. Published by Elsevier Inc. All rights reserved.)