학술논문
Restoration of Pregnancy Function Using a GT/PCL Biofilm in a Rabbit Model of Uterine Injury.
Document Type
Academic Journal
Author
Huang D; Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, P. R. China.; Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, P. R. China.; Liu J; The Affiliated Taian City Central Hospital of Qingdao University, Taian, P. R. China.; Yang J; The Affiliated Taian City Central Hospital of Qingdao University, Taian, P. R. China.; Liang J; Department of Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, P. R. China.; Zhang J; Shandong First Medical University, Tai'an, P. R. China.; Han Q; Shandong First Medical University, Jinan, P. R. China.; Yu J; Rizhao People's Hospital, Rizhao, P. R. China.; Yang T; Tai'an Maternal and Child Health Hospital, Tai'an, P. R. China.; Meng Q; The Affiliated Taian City Central Hospital of Qingdao University, Taian, P. R. China.; Steinberg T; Division of Oral Biotechnology, Center for Dental Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Li C; Center of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, P. R. China.; Institute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center, Shenzhen, P. R. China.; Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecologic Diseases, Shenzhen, P. R. China.; Chang Z; Shandong Agriculture University, Tai'an, P. R. China.
Source
Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101466659 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1937-335X (Electronic) Linking ISSN: 19373341 NLM ISO Abbreviation: Tissue Eng Part A Subsets: MEDLINE
Subject
Language
English
Abstract
Biomaterial scaffolds have been used successfully to promote the regenerative repair of small endometrial lesions in small rodents, providing partial restoration of gestational function. The use of rabbits in this study allowed us to investigate a larger endometrial tissue defect and myometrial injury model. A gelatin/polycaprolactone (GT/PCL) gradient-layer biofilm was sutured at the defect to guide the reconstruction of the original tissue structure. Twenty-eight days postimplantation, the uterine cavity had been restored to its original morphology, endometrial growth was accompanied by the formation of glands and blood vessels, and the fragmented myofibers of the uterine smooth muscle had begun to resemble the normal structure of the lagomorph uterine cavity, arranging in a circular luminal pattern and a longitudinal serosal pattern. In addition, the repair site supported both embryonic implantation into the placenta and normal embryonic development. Four-dimensional label-free proteomic analysis identified the cell adhesion molecules, phagosome, ferroptosis, rap1 signaling pathways, hematopoietic cell lineage, complement and coagulation cascades, tricarboxylic acid cycle, carbon metabolism, and hypoxia inducible factor (HIF)-1 signaling pathways as important in the endogenous repair process of uterine tissue injury, and acetylation of protein modification sites upregulated these signaling pathways.