학술논문
Interim Storage of Biospecimen at Satellite Collection Centers: Dewar and Cryotube Choice Are Important for Temporary Storage in Liquid Nitrogen.
Document Type
Academic Journal
Author
Niedermair T; Institute of Pathology, University Regensburg, University of Regensburg, Regensburg, Germany.; Central Biobank Regensburg, University Clinic and University of Regensburg, Regensburg, Germany.; Bhatt M; Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Nice Hospital Center, University Côte d'Azur, Nice, France.; Hospital-Integrated Biobank (BB-0033-00025), University Cote D'Azur, Nice, France.; Babel M; Institute of Pathology, University Regensburg, University of Regensburg, Regensburg, Germany.; Central Biobank Regensburg, University Clinic and University of Regensburg, Regensburg, Germany.; Feustel M; Institute of Pathology, University Regensburg, University of Regensburg, Regensburg, Germany.; Central Biobank Regensburg, University Clinic and University of Regensburg, Regensburg, Germany.; Mamilos A; Institute of Pathology, University Regensburg, University of Regensburg, Regensburg, Germany.; Schweikl H; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, Regensburg, Germany.; Ferstl G; Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, Regensburg, Germany.; Hofman P; Laboratory of Clinical and Experimental Pathology, FHU OncoAge, Nice Hospital Center, University Côte d'Azur, Nice, France.; Hospital-Integrated Biobank (BB-0033-00025), University Cote D'Azur, Nice, France.; Brochhausen C; Institute of Pathology, University Regensburg, University of Regensburg, Regensburg, Germany.; Central Biobank Regensburg, University Clinic and University of Regensburg, Regensburg, Germany.
Source
Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 101507284 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1947-5543 (Electronic) Linking ISSN: 19475543 NLM ISO Abbreviation: Biopreserv Biobank Subsets: MEDLINE
Subject
Language
English
Abstract
One major goal of biobanks is to provide the best possible biospecimen quality for research use. This can be achieved, notably in accredited structures, by using standardized procedures for collection, processing, and storage of biosamples and associated data. Since tissue samples of a clinical biobank are commonly collected at surgical theaters in satellite locations or hospitals in remote areas, adequate temporary storage of the biosample is mandatory to maintain optimal sample quality. In cases where immediate snap freezing of the collected material is possible, interim storage of the samples in portable dewars filled with liquid nitrogen (LN 2 ) is a widely used method. Therefore, the ideal dewar size and maximum storage time need to be considered to maintain an optimal biospecimen quality. In addition, the nature of the cryotube material is an important aspect for keeping the biosample safe while storing it in LN 2 . The objective of this study was to test different dewar vessels with respect to LN 2 volume and consumption and to analyze the impact of LN 2 contact on cryotube material through scanning electron microscopy.