학술논문

HIV-1 Gag-Pol Sequences from Ugandan Early Infections Reveal Sequence Variants Associated with Elevated Replication Capacity.
Document Type
Academic Journal
Author
Kapaata A; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Balinda SN; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Xu R; Emory University, Atlanta, GA 30322, USA.; Salazar MG; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Herard K; Emory University, Atlanta, GA 30322, USA.; Brooks K; Emory University, Atlanta, GA 30322, USA.; Laban K; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Hare J; Imperial College London, London SW7 2AZ, UK.; International AIDS Vaccine Initiative (IAVI), New York, NY 10004, USA.; Dilernia D; Emory University, Atlanta, GA 30322, USA.; Kamali A; IAVI, Nairobi 00202, Kenya.; Ruzagira E; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Mukasa F; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Gilmour J; Imperial College London, London SW7 2AZ, UK.; International AIDS Vaccine Initiative (IAVI), New York, NY 10004, USA.; Salazar-Gonzalez JF; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Yue L; Emory University, Atlanta, GA 30322, USA.; Cotten M; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.; Centre for Virus Research, MRC-University of Glasgow, Glasgow G61 1QH, UK.; Hunter E; Emory University, Atlanta, GA 30322, USA.; Kaleebu P; Medical Research Council, UVRI & LSTHM Uganda Research Unit, Plot 51-59, Entebbe, Uganda.
Source
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
Subject
Language
English
Abstract
The ability to efficiently establish a new infection is a critical property for human immunodeficiency virus type 1 (HIV-1). Although the envelope protein of the virus plays an essential role in receptor binding and internalization of the infecting virus, the structural proteins, the polymerase and the assembly of new virions may also play a role in establishing and spreading viral infection in a new host. We examined Ugandan viruses from newly infected patients and focused on the contribution of the Gag-Pol genes to replication capacity. A panel of Gag-Pol sequences generated using single genome amplification from incident HIV-1 infections were cloned into a common HIV-1 NL4.3 pol/env backbone and the influence of Gag-Pol changes on replication capacity was monitored. Using a novel protein domain approach, we then documented diversity in the functional protein domains across the Gag-Pol region and identified differences in the Gag-p6 domain that were frequently associated with higher in vitro replication.