학술논문
ppGpp influences protein protection, growth and photosynthesis in Phaeodactylum tricornutum.
Document Type
Academic Journal
Author
Avilan L; CNRS, BIP, UMR 7281, IMM FR 3479, Aix Marseille Univ, 31 Chemin Joseph Aiguier, Marseille, 13009, France.; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth, PO1 2DY, UK.; Lebrun R; Plate-forme Protéomique, Marseille Protéomique (MaP), IMM FR 3479, 31 Chemin Joseph Aiguier, Marseille, 13009, France.; Puppo C; CNRS, BIP, UMR 7281, IMM FR 3479, Aix Marseille Univ, 31 Chemin Joseph Aiguier, Marseille, 13009, France.; Citerne S; Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, Versailles, 78000, France.; Cuiné S; CEA, CNRS, UMR7265 BIAM, CEA Cadarache, Aix-Marseille Univ, Saint-Paul-lez Durance, 13108, France.; Li-Beisson Y; CEA, CNRS, UMR7265 BIAM, CEA Cadarache, Aix-Marseille Univ, Saint-Paul-lez Durance, 13108, France.; Menand B; CEA, CNRS, UMR7265 BIAM, Aix-Marseille Univ, Marseille, 13009, France.; Field B; CEA, CNRS, UMR7265 BIAM, Aix-Marseille Univ, Marseille, 13009, France.; Gontero B; CNRS, BIP, UMR 7281, IMM FR 3479, Aix Marseille Univ, 31 Chemin Joseph Aiguier, Marseille, 13009, France.
Source
Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE
Subject
Language
English
Abstract
Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta- and tetraphosphate ((p)ppGpp). In the model flowering plant Arabidopsis, ppGpp acts as a potent regulator of plastid gene expression and influences photosynthesis, plant growth and development. However, little is known about ppGpp metabolism or its evolution in other photosynthetic eukaryotes. Here, we studied the function of ppGpp in the diatom Phaeodactylum tricornutum using transgenic lines containing an inducible system for ppGpp accumulation. We used these lines to investigate the effects of ppGpp on growth, photosynthesis, lipid metabolism and protein expression. We demonstrate that ppGpp accumulation reduces photosynthetic capacity and promotes a quiescent-like state with reduced proliferation and ageing. Strikingly, using nontargeted proteomics, we discovered that ppGpp accumulation also leads to the coordinated upregulation of a protein protection response in multiple cellular compartments. Our findings highlight the importance of ppGpp as a fundamental regulator of chloroplast function across different domains of life, and lead to new questions about the molecular mechanisms and roles of (p)ppGpp signalling in photosynthetic eukaryotes.
(© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.)
(© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.)