학술논문
Preclinical evaluation of a COVID-19 vaccine candidate based on a recombinant RBD fusion heterodimer of SARS-CoV-2.
Document Type
Academic Journal
Author
Barreiro A; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Prenafeta A; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Bech-Sabat G; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Roca M; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Perozo Mur E; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; March R; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; González-González L; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Madrenas L; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Corominas J; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Fernández A; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Moros A; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Cañete M; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Molas M; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Pentinat-Pelegrin T; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Panosa C; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Moreno A; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Puigvert Molas E; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Pol Vilarrassa E; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Palmada J; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Garriga C; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Prat Cabañas T; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.; Iglesias-Fernández J; Nostrum Biodiscovery, Avenue de Josep Tarradellas, 8-10, 3-2, 08029 Barcelona, Spain.; Vergara-Alert J; Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Lorca-Oró C; Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Roca N; Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Fernández-Bastit L; Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Rodon J; Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Pérez M; Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Segalés J; Universitat Autònoma de Barcelona, CReSA (IRTA-UAB), Campus de la UAB, 08193 Cerdanyola del Vallès, Spain.; Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Cerdanyola del Vallès, Spain.; Pradenas E; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; Marfil S; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; Trinité B; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; Ortiz R; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; Clotet B; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; University of Vic-Central University of Catalonia (UVic-UCC), Vic, 08500 Catalonia, Spain.; Blanco J; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; University of Vic-Central University of Catalonia (UVic-UCC), Vic, 08500 Catalonia, Spain.; Díaz Pedroza J; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Ampudia Carrasco R; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Rosales Salgado Y; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Loubat-Casanovas J; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Capdevila Larripa S; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Prado JG; IrsiCaixa. AIDS Research Institute, Germans Trias i Pujol Research Institute (IGTP), Can Ruti Campus, UAB, 08916 Badalona, Spain.; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Barretina J; Comparative Medicine and Bioimage Centre of Catalonia, Germans Trias i Pujol Research Institute (CMCiB-IGTP), 08916 Badalona, Spain.; Sisteré-Oró M; Infection Biology Laboratory, Department of Experimental and Health Sciences (DCEXS), Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.; Cebollada Rica P; Infection Biology Laboratory, Department of Experimental and Health Sciences (DCEXS), Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.; Meyerhans A; Infection Biology Laboratory, Department of Experimental and Health Sciences (DCEXS), Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain.; Catalan Institution for Research and Advanced Studies (ICREA), Pg. Lluís Companys 23, 08010 Barcelona, Spain.; Ferrer L; HIPRA, Avda. La Selva, 135, Amer, 17170 Girona, Spain.
Source
Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Current COVID-19 vaccines have been associated with a decline in infection rates, prevention of severe disease, and a decrease in mortality rates. However, SARS-CoV-2 variants are continuously evolving, and development of new accessible COVID-19 vaccines is essential to mitigate the pandemic. Here, we present data on preclinical studies in mice of a receptor-binding domain (RBD)-based recombinant protein vaccine (PHH-1V) consisting of an RBD fusion heterodimer comprising the B.1.351 and B.1.1.7 SARS-CoV-2 variants formulated in SQBA adjuvant, an oil-in-water emulsion. A prime-boost immunisation with PHH-1V in BALB/c and K18-hACE2 mice induced a CD4 + and CD8 + T cell response and RBD-binding antibodies with neutralizing activity against several variants, and also showed a good tolerability profile. Significantly, RBD fusion heterodimer vaccination conferred 100% efficacy, preventing mortality in SARS-CoV-2 infected K18-hACE2 mice, but also reducing Beta, Delta and Omicron infection in lower respiratory airways. These findings demonstrate the feasibility of this recombinant vaccine strategy.
Competing Interests: Authors indicated as “1” are employees of HIPRA, a private pharmaceutical company that develops and manufactures vaccines. CReSA, IrsiCaixa, CMCiB-IGTP, UPF, and ICREA have received financial support from HIPRA. Two patent applications have been filed by HIPRA Scientific S.L.U. and Laboratorios HIPRA, S.A., on different SARS-CoV-2 vaccine candidates and SARS-CoV-2 subunit vaccines, including the recombinant RBD fusion heterodimer PHH-1V. Antonio Barreiro, Antoni Prenafeta, Luis González, Laura Ferrer, Ester Puigvert, Jordi Palmada, Teresa Prat, and Carme Garriga are the inventors of these patent applications.
(© 2023 The Author(s).)
Competing Interests: Authors indicated as “1” are employees of HIPRA, a private pharmaceutical company that develops and manufactures vaccines. CReSA, IrsiCaixa, CMCiB-IGTP, UPF, and ICREA have received financial support from HIPRA. Two patent applications have been filed by HIPRA Scientific S.L.U. and Laboratorios HIPRA, S.A., on different SARS-CoV-2 vaccine candidates and SARS-CoV-2 subunit vaccines, including the recombinant RBD fusion heterodimer PHH-1V. Antonio Barreiro, Antoni Prenafeta, Luis González, Laura Ferrer, Ester Puigvert, Jordi Palmada, Teresa Prat, and Carme Garriga are the inventors of these patent applications.
(© 2023 The Author(s).)