학술논문
Dark-purple rice extract modulates gut microbiota composition in acetic acid- and indomethacin-induced inflammatory bowel disease in rats.
Document Type
Academic Journal
Author
Thipart K; Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.; Division of Health and Applied Sciences (Pharmacology), Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.; Gruneck L; Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, Mueang, Thailand.; Phunikhom K; Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.; Sharpton TJ; Department of Microbiology, Oregon State University, Corvallis, OR, 97331, USA.; Department of Statistics, Oregon State University, Corvallis, OR, 97331, USA.; Sattayasai J; Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.; Popluechai S; Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, Mueang, Thailand. siam@mfu.ac.th.; School of Science, Mae Fah Luang University, Chiang Rai, Mueang, Thailand. siam@mfu.ac.th.
Source
Publisher: Springer Nature Country of Publication: Switzerland NLM ID: 9816585 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1905 (Electronic) Linking ISSN: 11396709 NLM ISO Abbreviation: Int Microbiol Subsets: MEDLINE
Subject
Language
English
Abstract
Ulcerative colitis (UC) and Crohn's disease (CD) are two major forms of inflammatory bowel disease (IBD). The disease has been linked with gut microbiota dysbiosis in which the balance of commensal communities is disrupted. Accumulating evidence demonstrates that treatment with biologically active compounds can modulate gut microbiota composition in animal models. Our previous work has also shown the beneficial effect of Luem Pua (LP) rice extract, which is rich in anthocyanins, on inflammation. However, its effect on gut microbiota is yet to be explored. In this study, we profiled fecal microbiota of acetic acid (AA)-induced UC and indomethacin (ID)-induced CD rat models with and without pretreatment with LP rice extract by 16S rRNA gene sequencing. The results showed that gut microbiota communities of rats were altered by both AA-induced UC and ID-induced CD. The relative abundances of beneficial bacteria, especially the Lachnospiraceae NK4A136 group and Lactobacillus, were decreased in the AA-induced UC model, while some opportunistic pathogens (Bacteroides, Escherichia/Shigella, Fusobacterium, and Veillonella) were raised by ID-induced CD. Interestingly, pretreatment with LP rice extract before AA-inducing UC in rats increased the proportion of the butyrate-producing bacteria (Lachnospiraceae NK4A136 group). The abundances of these beneficial bacteria and other SCFA-producing bacteria were unaffected by the indomethacin treatment with LP. Overall, our study revealed different impacts of AA-induced UC and ID-induced CD on changes in community composition and hinted at how LP may protect against UC by modifying the gut microbiota.
(© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
(© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)