학술논문
Hydroxychloroquine an Antimalarial Drug, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Multitargeting.
Document Type
Academic Journal
Author
Basrani ST; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; Gavandi TC; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; Patil SB; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; Kadam NS; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; iSERA Biological Pvt Ltd., MIDC Shirala, Dist., Sangli, Maharashtra, 41540, India.; Yadav DV; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; iSERA Biological Pvt Ltd., MIDC Shirala, Dist., Sangli, Maharashtra, 41540, India.; Chougule SA; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; Karuppayil SM; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India.; Jadhav AK; Department of Stem Cell and Regenerative Medicine and Medical Biotechnology, Centre for Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kadamwadi, Kolhapur, Maharashtra, 416003, India. ashujadhav09@gmail.com.
Source
Publisher: Microbiological Society Of Korea Country of Publication: Korea (South) NLM ID: 9703165 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1976-3794 (Electronic) Linking ISSN: 12258873 NLM ISO Abbreviation: J Microbiol Subsets: MEDLINE
Subject
Language
English
Abstract
Candida albicans is the primary etiological agent associated with candidiasis in humans. Unrestricted growth of C. albicans can progress to systemic infections in the worst situation. This study investigates the antifungal activity of Hydroxychloroquine (HCQ) and mode of action against C. albicans. HCQ inhibited the planktonic growth and yeast to hyphal form morphogenesis of C. albicans significantly at 0.5 mg/ml concentration. The minimum inhibitory concentrations (MIC 50 ) of HCQ for C. albicans adhesion and biofilm formation on the polystyrene surface was at 2 mg/ml and 4 mg/ml respectively. Various methods, such as scanning electron microscopy, exploration of the ergosterol biosynthesis pathway, cell cycle analysis, and assessment of S oxygen species (ROS) generation, were employed to investigate HCQ exerting its antifungal effects. HCQ was observed to reduce ergosterol levels in the cell membranes of C. albicans in a dose-dependent manner. Furthermore, HCQ treatment caused a substantial arrest of the C. albicans cell cycle at the G0/G1 phase, which impeded normal cell growth. Gene expression analysis revealed upregulation of SOD2, SOD1, and CAT1 genes after HCQ treatment, while genes like HWP1, RAS1, TEC1, and CDC 35 were downregulated. The study also assessed the in vivo efficacy of HCQ in a mice model, revealing a reduction in the pathogenicity of C. albicans after HCQ treatment. These results indicate that HCQ holds for the development of novel antifungal therapies.
(© 2024. The Author(s), under exclusive licence to Microbiological Society of Korea.)
(© 2024. The Author(s), under exclusive licence to Microbiological Society of Korea.)