학술논문
Assessment of Zn and Cu in piglets' liver and kidney: impact in fecal Enterococcus spp.?
Document Type
Academic Journal
Author
Donato MM; Faculdade de Medicina, Universidade de Coimbra, CIMAGO, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal.; Assis G; Laboratório de Controlo da Alimentação Animal, Unidade Estratégica de Investigação E Serviços, Tecnologia E Segurança Alimentar, Instituto Nacional de Investigação Agrária E Veterinária, I.P., Av. da República, Quinta Do Marquês, 2780-157, Oeiras, Portugal.; Cardoso O; Faculdade de Farmácia, Universidade de Coimbra, CERES, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal. ocardoso@ci.uc.pt.; Oliveiros B; Faculdade de Medicina, Universidade de Coimbra, LBIM, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal.; Faculdade de Medicina, Universidade de Coimbra, CIMAGO, I-CBR, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal.; Freitas A; Laboratório de Controlo da Alimentação Animal, Unidade Estratégica de Investigação E Serviços, Tecnologia E Segurança Alimentar, Instituto Nacional de Investigação Agrária E Veterinária, I.P., Av. da República, Quinta Do Marquês, 2780-157, Oeiras, Portugal.; Laboratório Nacional de Referência Para a Segurança Alimentar, Instituto Nacional de Investigação Agrária E Veterinária, I.P., Rua Dos Lágidos, Lugar da Madalena, 4485-655, Vairão, Vila Do Conde, Portugal.; Ramos F; REQUIMTE/LAQV, Rua Dom Manuel II, Apartado 55142, 4051-401, Porto, Portugal.; Faculdade de Farmácia, Universidade de Coimbra, Azinhaga de Santa Comba, 3000-548, Coimbra, Portugal.
Source
Publisher: Springer Country of Publication: Germany NLM ID: 9441769 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1614-7499 (Electronic) Linking ISSN: 09441344 NLM ISO Abbreviation: Environ Sci Pollut Res Int Subsets: MEDLINE
Subject
Language
English
Abstract
Zinc and copper have been used as growth promotors in alternative to antibiotics in pig's diet. The aim was the ascertainment of the Zn and Cu concentrations in piglets' liver and kidney and their impact in the reduced susceptibility to Zn, Cu, and antibiotics in enterococci, used as microbiota biomarker. Zn and Cu were determined in the livers and kidneys of 43 piglets slaughtered in Portugal, by flame atomic absorption spectrometry. Enterococci were isolated from feces for determining the identification of species (E. faecalis, E. faecium, and Enterococcus spp.); susceptibility to vancomycin, ciprofloxacin, linezolid, tigecycline, ampicillin, imipenem, and metals; and Cu tolerance genes. In piglets with Zn and Cu high or toxic levels, enterococci had reduced susceptibility to ions, reinforced by the presence of Cu tolerance genes and by resistance to antibiotics. The study relevance is to show the relationship between these metals' levels and decreased susceptibility to Cu, Zn, and antibiotics by enterococci. From the results, it could be supposed that the piglets were being fed with high doses of Zn and Cu which could select more resistant bacteria to both antibiotics and metals that could spread to environment and humans.
(© 2024. The Author(s).)
(© 2024. The Author(s).)