학술논문
Multidrug-resistant Klebsiella pneumoniae clinical isolates producing NDM- and OXA-type carbapenemase in Nepal.
Document Type
Academic Journal
Author
Takei S; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Tabe Y; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Miida T; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Hishinuma T; Department of Microbiology, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Khasawneh A; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Kirikae T; Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Sherchand JB; Department of Medical Microbiology, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.; Tada T; Department of Microbiology, Juntendo University Graduate School of Medicine, Tokyo, Japan. Electronic address: t-tada@juntendo.ac.jp.
Source
Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE
Subject
Language
English
Abstract
Objectives: The emergence of multidrug-resistant Klebsiella pneumoniae has become a serious problem in medical settings worldwide.
Methods: A total of 46 isolates of multidrug-resistant K. pneumoniae were obtained from 2 hospitals in Nepal from October 2018 to April 2019.
Results: Most of these isolates were highly resistant to carbapenems, aminoglycosides, and fluoroquinolones with the minimum inhibitory concentrations (MICs) of more than 64 µg/mL. These isolates harboured carbapenemase-encoding genes, including blaNDM-1 , bla NDM-5 , bla OXA-181 and bla OXA-232 , and 16S rRNA methyltransferase-encoding genes, including armA, rmtB, rmtC, and rmtF. Multilocus sequence typing revealed that 44 of 46 isolates were high-risk clones such as ST11 (2%), ST14 (4%), ST15 (11%), ST37 (2%), ST101 (2%), ST147 (28%), ST231 (13%), ST340 (4%), and ST395 (28%). In particular, ST395 isolates, which spread across medical settings in Nepal, co-harboured bla NDM-5 and rmtB on IncFII plasmids and co-harboured bla OXA-181/-232 and rmtF on ColKP3 plasmids. Several isolates harboured bla OXA-181 or bla NDM-5 on their chromosomes and multi-copies of bla NDM-1 or genes encoding 16S rRNA methyltransferases on their plasmids.
Conclusions: The presented study demonstrates that the high-risk clones of multidrug-resistant K. pneumoniae spread in a clonal manner across hospitals in Nepal.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Methods: A total of 46 isolates of multidrug-resistant K. pneumoniae were obtained from 2 hospitals in Nepal from October 2018 to April 2019.
Results: Most of these isolates were highly resistant to carbapenems, aminoglycosides, and fluoroquinolones with the minimum inhibitory concentrations (MICs) of more than 64 µg/mL. These isolates harboured carbapenemase-encoding genes, including bla
Conclusions: The presented study demonstrates that the high-risk clones of multidrug-resistant K. pneumoniae spread in a clonal manner across hospitals in Nepal.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)