학술논문
Isolation and identification of Wickerhamiella tropicalis from blood culture by MALDI-MS.
Document Type
Academic Journal
Author
Takei S; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Teramoto K; Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Analytical & Measurement Instruments Division, Shimadzu Corporation, Kyoto, Japan.; Fujimura J; Department of Pediatrics, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Fujiwara M; Department of Pediatrics, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Suzuki M; Department of General Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Fukui Y; Department of General Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Sekiguchi Y; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.; Kawakami T; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Chonan M; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Wakita M; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Horiuchi Y; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Miida T; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Naito T; Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of General Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Kirikae T; Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Tada T; Department of Microbiology, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Tabe Y; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Source
Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
Subject
Language
English
Abstract
Wickerhamiella is a genus of budding yeast that is mainly isolated from environmental samples, and 40 species have been detected. The yeast isolated from human clinical samples usually only contain three species: W. infanticola , W. pararugosa and W. sorbophila . In this study, we isolated W. tropicalis from a blood sample of a six-year-old female with a history of B-cell precursor lymphoblastic leukemia in Japan in 2022. Though the strain was morphologically identified as Candida species by routine microbiological examinations, it was subsequently identified as W. tropicalis by sequencing the internal transcribed spacer (ITS) of ribosomal DNA (rDNA). The isolate had amino acid substitutions in ERG11 and FKS1 associated with azole and echinocandin resistance, respectively, in Candida species and showed intermediate-resistant to fluconazole and micafungin. The patient was successfully treated with micafungin. Furthermore, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) detected three novel peaks that are specific for W. tropicalis , indicating that MALDI-MS analysis is useful for rapid detection of Wickerhamiella species in routine microbiological examinations.
Competing Interests: The Department of MALDI-TOF MS Practical Application Research at Juntendo University Graduate School of Medicine has been endowed partially by Shimadzu Corp. Kyoto, Japan to develop and validate new diagnostic technology and to conduct academic research through collaborations. ST, KT, TN, TK, and YT belong to the Department of MALDI-TOF MS Practical Application Research. Shimadzu Corp. provided MALDI-8020 and reagents for MALDI-MS analysis free of cost to YT. Author KT is employed by Shimadzu Corp. The study was performed by scientifically proper methods without any bias. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Takei, Teramoto, Fujimura, Fujiwara, Suzuki, Fukui, Sekiguchi, Kawakami, Chonan, Wakita, Horiuchi, Miida, Naito, Kirikae, Tada and Tabe.)
Competing Interests: The Department of MALDI-TOF MS Practical Application Research at Juntendo University Graduate School of Medicine has been endowed partially by Shimadzu Corp. Kyoto, Japan to develop and validate new diagnostic technology and to conduct academic research through collaborations. ST, KT, TN, TK, and YT belong to the Department of MALDI-TOF MS Practical Application Research. Shimadzu Corp. provided MALDI-8020 and reagents for MALDI-MS analysis free of cost to YT. Author KT is employed by Shimadzu Corp. The study was performed by scientifically proper methods without any bias. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Takei, Teramoto, Fujimura, Fujiwara, Suzuki, Fukui, Sekiguchi, Kawakami, Chonan, Wakita, Horiuchi, Miida, Naito, Kirikae, Tada and Tabe.)