학술논문
Evaluation of bone marrow aspirates using the automated hematology analyzer Sysmex XN-3000.
Document Type
Academic Journal
Author
Tsuchiya K; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Department of Next Generation Hematology Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Kimura K; Department of Next Generation Hematology Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Sysmex Corporation, Kobe, Japan.; Ai T; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Yamamoto T; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Nagasaka K; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Takemura H; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Edahiro Y; Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan.; Saito K; Department of Next Generation Hematology Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Horiuchi Y; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Misawa S; Department of Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan.; Takaku T; Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan.; Ando J; Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan.; Ando M; Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan.; Miida T; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Uchihashi K; Sysmex Corporation, Kobe, Japan.; Tabe Y; Department of Next Generation Hematology Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Source
Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 101300213 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1751-553X (Electronic) Linking ISSN: 17515521 NLM ISO Abbreviation: Int J Lab Hematol Subsets: MEDLINE
Subject
Language
English
Abstract
Introduction: This study evaluated the feasibility of the Sysmex XN-3000 automated hematology analyzer for the assessment of total nucleated cells (TNC) and bone marrow (BM) cell density in routine bone marrow aspiration (BMA) samples.
Methods: A total of 54 BMA samples from 39 hematological patients were evaluated. The number of megakaryocytes was calculated by a specific gating algorithm using the body fluid mode of the WBC differential (WDF) channel. Lipid contents were calculated through a newly developed algorithm utilizing the WDF channel. The ratio of lipid particles over TNCs by the WNR channel was compared with the BM cellularity assessed by the BM biopsy. The myeloid/erythroid (M/E) ratio was calculated by measuring the number of myeloid cells in the WDF channel and the number of nucleated red blood cells (NRBCs) in the WNR channel.
Results: XN-3000 counts and microscopic results showed a linear correlation in TNC (R2 = .98, p < .001), megakaryocytes (R 2 = .59, p = .002), NRBC (R 2 = .84, p < .001), and M/E ratio (R 2 = .59, p < .001). There were significant differences in the lipid/TNC ratios of hypercellular, normocellular, and hypocellular BMs measured by XN-3000 (p < .001). Receiver-operating characteristic analysis detected cut-off values of the lipid/TNC ratio of >0.4054 for hypoplasia and <0.157 for hyperplasia. The sensitivity and specificity for hypoplasia were 100% and 88%, and for hyperplasia were 89% and 86%, respectively.
Conclusion: XN-3000 provides a quantitative assessment of BM cellularity, supporting the qualitative assessment by myelogram and BM biopsy.
(© 2023 The Authors. International Journal of Laboratory Hematology published by John Wiley & Sons Ltd.)
Methods: A total of 54 BMA samples from 39 hematological patients were evaluated. The number of megakaryocytes was calculated by a specific gating algorithm using the body fluid mode of the WBC differential (WDF) channel. Lipid contents were calculated through a newly developed algorithm utilizing the WDF channel. The ratio of lipid particles over TNCs by the WNR channel was compared with the BM cellularity assessed by the BM biopsy. The myeloid/erythroid (M/E) ratio was calculated by measuring the number of myeloid cells in the WDF channel and the number of nucleated red blood cells (NRBCs) in the WNR channel.
Results: XN-3000 counts and microscopic results showed a linear correlation in TNC (R
Conclusion: XN-3000 provides a quantitative assessment of BM cellularity, supporting the qualitative assessment by myelogram and BM biopsy.
(© 2023 The Authors. International Journal of Laboratory Hematology published by John Wiley & Sons Ltd.)