학술논문
In vitro antimicrobial and antioxidant activities, essential oil composition, and in silico molecular modeling analysis of secondary metabolites from roots of Verbascum sinaiticum .
Document Type
Academic Journal
Author
Tegegn G; Department of Applied Chemistry, 125545 School of Applied Natural Science, Adama Science and Technology University , P.O. Box 1888, Adama, Ethiopia.; Melaku Y; Department of Applied Chemistry, 125545 School of Applied Natural Science, Adama Science and Technology University , P.O. Box 1888, Adama, Ethiopia.; Aliye M; Department of Applied Chemistry, 125545 School of Applied Natural Science, Adama Science and Technology University , P.O. Box 1888, Adama, Ethiopia.; Abebe A; Traditional and Modern Drug Research and Development Directorate, 70605 Armauer Hansen Research Institute , P.O. Box 1242, Addis Ababa, Ethiopia.; Abdissa N; Traditional and Modern Drug Research and Development Directorate, 70605 Armauer Hansen Research Institute , P.O. Box 1242, Addis Ababa, Ethiopia.; Meresa A; Traditional and Modern Drug Research and Development Directorate, 70605 Armauer Hansen Research Institute , P.O. Box 1242, Addis Ababa, Ethiopia.; Degu S; Traditional and Modern Drug Research and Development Directorate, 70605 Armauer Hansen Research Institute , P.O. Box 1242, Addis Ababa, Ethiopia.; Hunsen M; Department of Chemistry, 3475 Kenyon College , Gambier, OH 43022, USA.; Hussein AA; Department of Chemistry, 70683 Cape Peninsula University of Technology , Bellville Campus, Bellville 7535, Western Cape, South Africa.; Endale M; Traditional and Modern Drug Research and Development Directorate, 70605 Armauer Hansen Research Institute , P.O. Box 1242, Addis Ababa, Ethiopia.
Source
Publisher: De Gruyter Country of Publication: Germany NLM ID: 8912155 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1865-7125 (Electronic) Linking ISSN: 03410382 NLM ISO Abbreviation: Z Naturforsch C J Biosci Subsets: MEDLINE
Subject
Language
English
Abstract
Verbascum sinaiticum is locally used to treat wound, stomachache, viral infection, cancer, sunstroke fever, abdominal colic, diarrhea, hemorrhage, anthrax, and hepatitis. The objective of this study was to identify the compounds and to evaluate the antimicrobial and antioxidant activity of the extracts and isolated compounds from V. sinaiticum . The 1 H-NMR, 13 C-NMR, and DEPT-135 were used to elucidate the structures of isolated compounds. Essential oils were extracted by hydrodistillation method and their chemical analyses were performed by GC-MS. The broth microdilution method was used to evaluate the antimicrobial activity. The radical scavenging activity of the extracts and isolated compounds were evaluated using DPPH method. Silica gel column chromatographic separation of root extracts afforded seven known compounds: 3'-(4''-methoxy phenyl)-3'-oxo-propionyl hexadecanoate ( 1 ), harpagoside ( 2 ), pulverulentoside I ( 3 ), scrophuloside B4 ( 4 ), scropolioside A ( 5 ), scropolioside-D2 ( 6 ), and harpagide 6- O -β-glucoside ( 7 ), which are all reported from this species for the first time. The EO extracts from leaves and roots were the most susceptible to Streptococcus agalactiae , with a 2 mg/mL MIC. The EO from roots was effective against Candida albicans and Trichophyton mentagrophytes , with a MIC of 8 mg/mL. The MeOH and CH 2 Cl 2 /CH 3 OH (1:1) root extracts showed the maximum activity against S. epidermidis with MIC values of 0.25 mg/mL. The strongest antibacterial effects were demonstrated against Staphylococcus epidermidis , which exhibited a 0.0625 mg/mL MIC for compound 1 . The strongest radical scavenging activity was exhibited by the methanol extract (IC 50 = 3.4 μg/mL), and compounds 4 , 6 , 5 , 3 , 7 , and 2 with IC 50 values of 3.2, 3.38, 3.6, 3.8, 4.2, and 4.7 μg/mL, respectively, in comparison with ascorbic acid (IC 50 = 1.3 μg/mL). The results of the molecular docking analysis of compounds revealed minimal binding energies range from -38.5 to -43.1 kJ/mol, -33.1 to -42.7 kJ/mol, -34.7 to -39.3.7 kJ/mol, -25.5 to -37.6 kJ/mol against human myeloperoxidase (PDB ID: 1DNU), murA enzyme (PDB ID: 1UAE), human topoisomerase II β (PDB ID: 4fm9), S. epidermidis FtsZ (PDB number: 4M8I) proteins, respectively. The docking results and the in vitro antibacterial activity are in good agreement. These findings show that the isolated compounds 2 - 7 can act as potential antioxidants and strong antibacterials against Staphylococcus aureus and S. epidermidis . As a result, V. sinaiticum root extracts have the potential to be effective in treating diseases caused by bacteria and free radicals, as long as further investigation has been suggested for the ultimate decision of this plant's potential candidate.
(© 2024 Walter de Gruyter GmbH, Berlin/Boston.)
(© 2024 Walter de Gruyter GmbH, Berlin/Boston.)