학술논문
Proapoptotic and proautophagy effect of H1-receptor antagonist desloratadine in human glioblastoma cell lines.
Document Type
Academic Journal
Author
Vidicevic-Novakovic S; School of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Belgrade, Serbia.; Stanojevic Z; School of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Belgrade, Serbia.; Tomonjic N; School of Medicine, Institute of Rheumatology, University of Belgrade, Belgrade, Serbia.; Karapandza K; School of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Belgrade, Serbia.; Zekovic J; School of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Belgrade, Serbia.; Martinovic T; School of Medicine, Institute of Histology and Embryology, University of Belgrade, Belgrade, Serbia.; Grujicic D; Clinic of Neurosurgery, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Belgrade, Serbia.; Ilic R; Clinic of Neurosurgery, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Belgrade, Serbia.; Raicevic S; Clinic of Neurosurgery, Clinical Centre of Serbia, School of Medicine, University of Belgrade, Belgrade, Serbia.; Tasic J; School of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Belgrade, Serbia. jelena.tasic@med.bg.ac.rs.; Isakovic A; School of Medicine, Institute of Medical and Clinical Biochemistry, University of Belgrade, Belgrade, Serbia.
Source
Publisher: Springer Country of Publication: United States NLM ID: 9435512 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-131X (Electronic) Linking ISSN: 13570560 NLM ISO Abbreviation: Med Oncol Subsets: MEDLINE
Subject
Language
English
Abstract
Glioblastomas are aggressive and usually incurable high-grade gliomas without adequate treatment. In this study, we aimed to investigate the potential of desloratadine to induce apoptosis/autophagy as genetically regulated processes that can seal cancer cell fates. All experiments were performed on U251 human glioblastoma cell line and primary human glioblastoma cell culture. Cytotoxic effect of desloratadine was investigated using MTT and CV assays, while oxidative stress, apoptosis, and autophagy were detected by flow cytometry and immunoblot. Desloratadine treatment decreased cell viability of U251 human glioblastoma cell line and primary human glioblastoma cell culture (IC50 value 50 µM) by an increase of intracellular reactive oxygen species and caspase activity. Also, desloratadine decreased the expression of main autophagy repressor mTOR and its upstream activator Akt and increased the expression of AMPK. Desloratadine exerted dual cytotoxic effect inducing both apoptosis- and mTOR/AMPK-dependent cytotoxic autophagy in glioblastoma cells and primary glioblastoma cell culture.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)