학술논문
Pulsed electromagnetic fields reduce acute inflammation in the injured rat-tail intervertebral disc.
Document Type
Academic Journal
Author
Chan AK; Department of Neurological Surgery University of California San Francisco San Francisco California.; Tang X; Department of Orthopaedic Surgery University of California San Francisco California.; Mummaneni NV; Department of Orthopaedic Surgery University of California San Francisco California.; Coughlin D; Department of Orthopaedic Surgery University of California San Francisco California.; Liebenberg E; Department of Orthopaedic Surgery University of California San Francisco California.; Ouyang A; Department of Orthopaedic Surgery University of California San Francisco California.; Dudli S; Department of Orthopaedic Surgery University of California San Francisco California.; Lauricella M; Department of Orthopaedic Surgery University of California San Francisco California.; Zhang N; Orthofix Inc. Lewisville Texas.; Waldorff EI; Orthofix Inc. Lewisville Texas.; Ryaby JT; Orthofix Inc. Lewisville Texas.; Lotz JC; Department of Orthopaedic Surgery University of California San Francisco California.
Source
Publisher: Wiley Periodicals, Inc Country of Publication: United States NLM ID: 101722350 Publication Model: eCollection Cited Medium: Internet ISSN: 2572-1143 (Electronic) Linking ISSN: 25721143 NLM ISO Abbreviation: JOR Spine Subsets: PubMed not MEDLINE
Subject
Language
English
Abstract
Pro-inflammatory cytokines are recognized contributors to intervertebral disc (IVD) degeneration and discogenic pain. We have recently reported the anti-inflammatory effect of pulsed electromagnetic fields (PEMF) on IVD cells in vitro. Whether these potentially therapeutic effects are sufficiently potent to influence disc health in vivo has not been demonstrated. We report here the effect of PEMF on acute inflammation arising from a rat-tail IVD injury model. Disc degeneration was induced by percutaneously stabbing the Co6-7, Co7-8, and Co8-9 levels using a 20-gauge needle. Seventy-two (72) rats were divided into three groups: sham control, needle stab, needle stab+PEMF. Treated rats were exposed to PEMF immediately following surgery and for either 4 or 7 days (4 hr/d). Stab and PEMF effects were evaluated by measuring inflammatory cytokine gene expression (RT-PCR) and protein levels (ELISA assay), anabolic and catabolic gene expression (RT-PCR), and histologic changes. We observed in untreated animals that at day 7 after injury, inflammatory cytokines (interleukin [IL]-6, tumor necrosis factor α, and IL-1β) were significantly increased at both gene and protein levels ( P < .05). Similarly, catabolic factors (MMP [metalloproteinases]-2, MMP-13 and the transcriptional factor NF-kβ gene expression) were significantly increased ( P < .05). At day 7, PEMF treatment significantly inhibited inflammatory cytokine gene and protein expression induced by needle stab injury ( P < .05). At day 4, PEMF downregulated FGF-1 and upregulated MMP-2 compared to the stab-only group. These data demonstrate that previously reported anti-inflammatory effects of PEMF on disc cells carry over to the in vivo situation, suggesting potential therapeutic benefits. Though we observed an inhibitory effect of PEMF on acute inflammatory cytokine expression, a consistent effect was not observed for acute changes in disc histology and anabolic and catabolic factor expression. Therefore, these findings should be further investigated in studies of longer duration following needle-stab injury.
(© 2019 The Authors. JOR Spine published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society.)
(© 2019 The Authors. JOR Spine published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society.)